Table 1 Sequence of single guide RNA (gRNA), forward and reverse PCR primers (PCR-fw and PCR-rev), and primers used for sequencing (p1 and p2)
From: Death receptor-based enrichment of Cas9-expressing cells
Gene | Type/Name | Sequence | Genomic location (Assembly Dec. 2013 GRCh38/hg38) |
|---|---|---|---|
IRF3 encoded on (−) strand of genome | gRNA-1 | GCCACTGGTGCATATGTTCC | 19:49663483–49663502 (−) |
gRNA-2 | CCACTGGTGCATATGTTCCC | 19:49663482–49663501 (−) | |
gRNA-3 | ATAAGCCAGACCTGCCAACC | 19:49663455–49663474 (−) | |
PCR-fw | TTCTCACCTGGGTATCAGAAGTA | 19:49663181–49663203 (+) | |
PCR-rev | TGAGGTTCCTAACTACCGAATTA | 19:49664294–49664316 (−) | |
p1 | TGTCTGGCTGGGAAAAGTC | 19:49663232–49663250 (+) | |
p2 | CTGTAATCCCAGCACTTTG | 19:49663960–49663978 (−) | |
TLR3 encoded on (+) strand of genome | gRNA-1 | ACATTAGATCTGTCTCATAA | 4:186078849–186078868 (+) |
gRNA-2 | ATTAGGAACTCAGGTTCAGC | 4:186078887–186078906 (+) | |
gRNA-3 | GGCTTGTCATCTACAAAATT | 4:186078870–186078889 (+) | |
PCR-fw | TGTGTTTGATAAGCCATGTGA | 4:186078463–186078483 (+) | |
PCR-rev | GGAGGCTAGAGAGGGAGAAC | 4:186079465–186079484 (−) | |
p1 | AATCCTTCCTACAATGG | 4:186078618–186078634 (+) | |
p2 | AGGATTGCTGGAAGACAGG | 4:186079129–186079147 (−) | |
p65 encoded on (−) strand of genome | gRNA-1 | TCAATGGCTACACAGGACCA | 11:65661815–65661834 (−) |
gRNA-2 | AGGGACAGTGCGCATCTCCC | 11:65661796–65661815 (−) | |
gRNA-3 | AGCTTGTAGGAAAGGACTGC | 11:65661737–65661756 (−) | |
PCR-fw | AATGGTTTTCTTCCTCAAACAA | 11:65661392–65661413 (+) | |
PCR-rev | CTTAGTTTCACCGCAGGTTCTA | 11:65662331–65662352 (−) | |
p1 | GTATCCCCTGGAACTCATC | 11:65661487–65661505 (+) | |
p2 | TGTTCCCCCTCATCTTC | 11:65662186–65662202 (−) | |
gfp | gRNA | AAGGGCGAGGAGCTGTTCAC | - |