Fig. 2From: Casamino acids facilitate the secretion of recombinant dengue virus serotype-3 envelope domain III in Pichia pastoris Effect of induction temperature and casamino acids supplementation on the localization of expressed EDIII. Expression was tested at six CA concentrations (0, 0.25, 0.5, 1.0, 1.5, 2.0 % w/v) for 6 days at 20 °C (a) and 30 °C (b). EDIII, viz. secreted (green) and intracellular (red) levels were determined using immunoassay and value inside each bar represents EDIII titers in mg/L broth. Similar patterns were observed from culture supernatant (secretory EDIII) and cell mass (intracellular EDIII) from two independent inductionsBack to article page