Fig. 1From: Casamino acids facilitate the secretion of recombinant dengue virus serotype-3 envelope domain III in Pichia pastoris Coomassie stained SDS-PAGE showing secreted EDIII. Presence of secretory EDIII was determined in the supernatant of cultures induced in the absence (CA 0 %) or presence of different concentrations of casamino acids (CA 0.25 % to 2.0 % w/v) supplemented BMM media from day 0 to day 6 at 20 °C (a) and 30 °C (b). For both panels, similar trends were observed in two independent induction experiments (Additional file 9)Back to article page