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Table 1 Effects of various reagents on the activity of EstS and mutant

From: Characterization of a cold-active esterase from Serratia sp. and improvement of thermostability by directed evolution

Reagents

Relative activity (%)

EstS

1-D5

1 mM

5 mM

1 mM

5 mM

Control

9.9 ± 0.2 U/ml

8.6 ± 0.5 U/ml

8.1 ± 3.2 U/ml

8.1 ± 3.2 U/ml

Mg2+

121.8 ± 1.3

106.5 ± 5.9

95.0 ± 4.4

98.3 ± 1.4

Sr2+

102.0 ± 1.7

105.3 ± 2.3

94.2 ± 4.4

101.1 ± 1.1

Ba2+

109.7 ± 5.7

107.6 ± 7.4

97.2 ± 3.1

103.2 ± 5.3

Zn2+

83.1 ± 3.6

71.6 ± 4.1

39.9 ± 6.3

34.2 ± 4.6

Mn2+

120.7 ± 4.0

114.9 ± 4.4

100.1 ± 7.3

106.2 ± 0.8

Cu2+

87.6 ± 4.2

52.1 ± 4.0

97.0 ± 7.0

73.9 ± 3.6

Ca2+

101.1 ± 1.3

106.2 ± 5.4

98.6 ± 3.5

101.4 ± 3.4

EDTA

96.3 ± 3.6

96.7 ± 1.0

99.1 ± 4.0

97.9 ± 2.6

PMSF

96.4 ± 3.5

89.8 ± 5.0

96.6 ± 4.7

54.8 ± 3.6

  1. Data are given as mean values ± SD. Activity was measured after adding 1 or 5 mM of various reagents into the reaction mixture. The activity without any additives was defined as 100 %
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