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Fig. 2 | BMC Biotechnology

Fig. 2

From: Characterization of a cold-active esterase from Serratia sp. and improvement of thermostability by directed evolution

Fig. 2

SDS-PAGE analysis of purified EstS protein. M: Protein molecular weight marker; 1: Uninduced cell lysate of E. coli BL21 (DE3) harboring pGEX-6P-1; 2: IPTG-induced of cell lysate of E. coli BL21 (DE3) harboring pGEX-6P-1; 3: Uninduced cell lysate of E. coli BL21 (DE3) harboring pGEX-6P-estS; 4: IPTG-induced of cell lysate of E. coli BL21 (DE3) harboring pGEX-6P-estS; 5: Purified EstS. The protein GST-EstS is indicated by arrow

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