Fig. 1

Multiple alignments of EstS and other four esterases. The four esterases are Est2 [PDB: 1EVQ_A] from Alicyclobacillus Acidocaldarius, PestE [PDB: 2YH2_A] from Pyrobaculum calidifontis, Este1 [PDB: 2C7B_A] from a metagenomic library and Lpest1 [PDB: 4C88_A] from Lactobacillus Plantarum. The identical and conserved residues are shaded. The conserved G–X–S–X–G motif and the catalytic triad (Ser, Asp, and His) were indicated by red box and black triangle, respectively