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Fig. 5 | BMC Biotechnology

Fig. 5

From: Identification and characterization of a novel stress-responsive outer membrane protein Lip40 from Actinobacillus pleuropneumoniae

Fig. 5

Expression changes of Lip40 protein in the outer-membrane fraction. a SDS-PAGE. Actinobacillus pleuropneumoniae outer-membrane samples were extracted from cells after they were treated with heating, anaerobic conditions, or cold stimulation, or with normal conditions. Equal amounts (66 μg) of outer-membrane proteins were analyzed with SDS-PAGE. b Western blotting analysis. The procedures were as described above. M, prestained protein ladder; lane 1, high temperature (42 °C); lane 2, anaerobic conditions; lane 3, low temperature (16 °C); lane 4, normal conditions (37 °C). c Intensity of the bands was quantified with the Quantity One software. Relative expression levels were obtained by normalizing the intensity of stress-treated samples to that of untreated samples in each assay. Double asterisks (**) indicate a significant difference (P < 0.01) compared with the stress-treated samples (Student’s t test)

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BMC Biotechnology

ISSN: 1472-6750

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