From: Development of an enzyme linked immunosorbent assay for detection of cyathane diterpenoids
Spiked concentration [μg mL-1] | ELISA (n = 3) | HPLC-DAD (n = 2) | ||
---|---|---|---|---|
Detected [μg mL-1] | Recovery [%] | Detected [μg mL-1] | Recovery [%] | |
10.010 | out of calibration | - | 10.50 ± 0.05 | 104.8% |
5.005 | out of calibration | - | 5.26 ± 0.10 | 105.1% |
2.002 | 1.949 ± 0.219 | 97.3% | 2.03 ± 0.08 | 101.5% |
1.001 | 1.048 ± 0.199 | 104.7% | 0.96 ± 0.02 | 95.8% |
0.501 | 0.630 ± 0.291 | 125.9% | 0.50 ± 0.00 | 99.6% |
0.400 | 0.478 ± 0.062 | 119.3% | 0.44 ± 0.02 | 110.2% |
0.200 | 0.252 ± 0.026 | 113.0% | n.d. | - |
0.020 | 0.021 ± 0.001 | 102.8% | n.d. | - |
0.001 | n.d* | - | n.d. | - |