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Table 1 List and characteristics of sample preps from HeLa and ZR-75-1 cell pellets.

From: Measuring microRNAs: Comparisons of microarray and quantitative PCR measurements, and of different total RNA prep methods

Cell Line

Prep Type

No. of Preps

RNA Yield Mean (SD)

260:280 Mean (SD)

260:230 Mean (SD)

RIN Mean (SD)

HeLa

TRIzol

10

45.59 (7.17)

1.89 (0.0406)

1.28 (0.208)

9.9 (0.14)

HeLa

mirVana

8

43.16 (12.01)

1.96 (0.0364)

1.36 (0.323)

9.9 (0.11)

HeLa

miRNeasy

11

34.07 (5.40)

2.08 (0.00874)

1.97 (0.389)

9.9 (0.11)

ZR-75-1

TRIzol

4

23.52 (1.39)

1.84 (0.0432)

0.815 (0.0759)

9.8 (0.075)

ZR-75-1

mirVana

4

16.92 (5.84)

2.01 (0.0356)

1.28 (0.361)

9.1 (0.17)

ZR-75-1

miRNeasy

4

18.37 (1.93)

2.05 (0.0311)

1.87 (0.337)

9.8 (0.050)

  1. The number of individual preps performed using the indicated total RNA prep method and cell lines are shown, as are the mean and standard deviations (in parentheses) of the total RNA yields (in micrograms), 260:280 ratios, 260:230 ratios, and RIN numbers for all the preps done with the same method in each cell type. Preps started with either 5 × 106 cells (HeLa) or 1 × 107 cells (ZR-75-1).
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BMC Biotechnology

ISSN: 1472-6750

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