From: Critical evaluation of HPV16 gene copy number quantification by SYBR green PCR
Cell line | Ploidy | Southern analysis Copy numbers per cell | NA6 qPCR analysis | |||
---|---|---|---|---|---|---|
 |  |  | E2 copies per diploid genome | E6 copies per diploid genome | Viral load per cell | Ratio of E2/E6 (range) |
C33A | Near triploid | HPV16 negative | 0 | 0 | 0 | 0.00 |
W12.Ser1p16 | Diploid | Episomal (approx 100 copies) | 148 | 143 | 143 | 1.04 (0.76–1.32) |
W12.Ser1p57 | Tetraploid | Integrated (approx 1 copy) | 0.2 | 0.3 | 1 | 0.72 (0.57–3.82) |
SiHa | Triploid | Integrated (2–3 copies) | 0.7 | 3.4 | 5 | 0.20 (0.11–0.24) |
CaSki | Tetraploid | Integrated (>1000 copies) | 2,272 | 869 | 1,738 | 2.61 (1.37–3.46) |