From: Critical evaluation of HPV16 gene copy number quantification by SYBR green PCR
Template DNA per μl | Expected quantification in 2 μl | Observed quantification with percent change over expected | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
 | E2 | E6 | HMBS | E2/E6 | E6 per diploid genome | E2 | E6 | HMBS | E2/E6 | E6 per diploid genome | |||
51 ng gDNA 10 pg pSP64 HPV16 | 760,000 | 760,000 | 28,571 | 1 | 53 | 893,063 | +17.5% | 616,115 | -18.9% | 49,816 | +74.4% | 1.4 | 25 |
11 ng gDNA 2.16 pg pSP64 HPV16 | 163,922 | 163,922 | 6,163 | 1 | 53 | 208,404 | +27.1% | 172,423 | +5.2% | 7,437 | +20.7% | 1.2 | 46 |
3 ng gDNA 60 fg pSP64 HPV16 | 44,665 | 44,665 | 1,679 | 1 | 53 | 26,956 | -39.7% | 40,597 | -9.1% | 1,047 | +37.6% | 0.7 | 78 |