From: Critical evaluation of HPV16 gene copy number quantification by SYBR green PCR
E6 | Correlation Coefficient (R2) | Primer Efficiency (E) | Number of molecules at threshold (Nt) | E6 gene copy numbers over a range of theoretical crossing points | ||||
---|---|---|---|---|---|---|---|---|
 |  |  |  | 10 | 15 | 20 | 25 | 30 |
Final calibration curve for E6 | 0.9963 | 95.01% | 3.3806E+11 | 4.25E+08 | 1.51E+07 | 5.34E+05 | 1.89E+04 | 6.72E+02 |
Replicate 1 | 0.9988 | 87.79% | 1.43162E+11 | 2.62E+08 | 1.12E+07 | 4.81E+05 | 2.06E+04 | 8.82E+02 |
Replicate 2 | 0.9909 | 101.86% | 5.85431E+11 | 5.21E+08 | 1.56E+07 | 4.64E+05 | 1.39E+04 | 4.13E+02 |
Replicate 3 | 0.9982 | 96.56% | 3.68007E+11 | 4.28E+08 | 1.46E+07 | 4.97E+05 | 1.69E+04 | 5.77E+02 |
Replicate 4 | 0.9973 | 93.84% | 2.55641E+11 | 3.41E+08 | 1.25E+07 | 4.56E+05 | 1.67E+04 | 6.09E+02 |
 |  |  | Coefficient of variation of replicates | 28.72% | 14.58% | 3.83% | 16.27% | 31.32% |
 |  |  | Mean copy number of replicates | 3.88E+08 | 1.35E+07 | 4.74E+05 | 1.70E+04 | 6.20E+02 |
E2 | Correlation Coefficient (R 2 ) | Primer Efficiency (E) | Number of molecules at threshold (Nt) | E2 gene copy numbers over a range of theoretical crossing points | ||||
 |  |  |  | 10 | 15 | 20 | 25 | 30 |
Final calibration curve for E2 | 0.9945 | 90.86% | 2.42762E+11 | 3.79E+08 | 1.49E+07 | 5.90E+05 | 2.33E+04 | 9.02E+02 |
Replicate 1 | 0.9887 | 88.55% | 1.92376E+11 | 3.39E+08 | 1.42E+07 | 5.96E+05 | 2.50E+04 | 0.9887 |
Replicate 2 | 0.9945 | 93.10% | 2.8539E+11 | 3.96E+08 | 1.48E+07 | 5.49E+05 | 2.05E+04 | 0.9945 |
Replicate 3 | 0.9973 | 92.70% | 3.11657E+11 | 4.41E+08 | 1.66E+07 | 6.25E+05 | 2.35E+04 | 0.9973 |
Replicate 4 | 0.9976 | 89.07% | 1.81623E+11 | 3.11E+08 | 1.29E+07 | 5.33E+05 | 2.21E+04 | 0.9976 |
 |  |  | Coefficient of variation of replicates | 15.69% | 10.60% | 7.36% | 8.57% | 13.06% |
 |  |  | Mean copy number of replicates | 3.72E+08 | 1.46E+07 | 5.76E+05 | 2.28E+04 | 9.02E+02 |
HMBS | Correlation Coefficient (R 2 ) | Primer Efficiency (E) | Number of molecules at threshold (Nt) | HMBS gene copy numbers over a range of theoretical crossing points | ||||
 |  |  |  | 10 | 15 | 20 | 25 | 30 |
Final calibration curve for HMBS | 0.9970 | 91.05% | 2.28115E+11 | 3.52E+08 | 1.38E+07 | 5.44E+05 | 2.14E+04 | 8.40E+02 |
Replicate 1 | 0.9951 | 89.10% | 1.333E+11 | 2.28E+08 | 9.43E+06 | 3.90E+05 | 1.61E+04 | 6.67E+02 |
Replicate 2 | 0.9985 | 95.39% | 3.52583E+11 | 4.35E+08 | 1.53E+07 | 5.36E+05 | 1.88E+04 | 6.60E+02 |
Replicate 3 | 0.9990 | 90.43% | 2.4809E+11 | 3.96E+08 | 1.58E+07 | 6.31E+05 | 2.52E+04 | 1.01E+03 |
Replicate 4 | 0.9953 | 89.26% | 1.78485E+11 | 3.03E+08 | 1.25E+07 | 5.13E+05 | 2.11E+04 | 8.71E+02 |
 |  |  | Coefficient of variation of replicates | 27.36% | 22.14% | 19.17% | 18.93% | 20.99% |
 |  |  | Mean copy number of replicates | 3.40E+08 | 1.32E+07 | 5.18E+05 | 2.03E+04 | 8.01E+02 |