It is also important to note that it is essential to fix the fluorescence threshold (Ft) to a single value across all runs, in order to avoid artificial quantitative variances.
The mathematics of standard curve construction and evaluation of quantitative variance generated by standard curves are addressed in detail in an earlier study:
Rutledge RG, Côté C: Mathematics of quantitative kinetic PCR and the application of standard curves.
Equation 2 is incorrect
23 October 2008
Equation 2 should read:
E = 10-Slope - 1
It is also important to note that it is essential to fix the fluorescence threshold (Ft) to a single value across all runs, in order to avoid artificial quantitative variances.
The mathematics of standard curve construction and evaluation of quantitative variance generated by standard curves are addressed in detail in an earlier study:
Rutledge RG, Côté C: Mathematics of quantitative kinetic PCR and the application of standard curves.
Nucleic Acids Res 2003, 31(16):e93.
Competing interests
I have no competing interests.