Figure 6From: Successful elimination of non-neural cells and unachievable elimination of glial cells by means of commonly used cell culture manipulations during differentiation of GFAP and SOX2 positive neural progenitors (NHA) to neuronal cellsSubcloning method revealed undifferentiated NHA cells as non-committed homogenous cell population. Immunocytochemistry results show: co-expression of GFAP and MAP2 in undifferentiated NHA cells passage 0 seeded at a very low density (5 000 cells/cm2) (a); equal "non-colonial" distribution of cells representing phenotype [MAP2+, CD44-] and phenotype [CD44+, MAP2-] obtained as a result of neural differentiation of NHA cells which previously reached confluence in medium with serum (b); ability of NHA cells to form colonies obtained as a result of neural differentiation of non-confluent cells (c).Back to article page