Figure 3From: Human IL-12 p40 as a reporter gene for high-throughput screening of engineered mouse embryonic stem cellsAnalysis of the CMV and PGK promoters in BHK-21, and COS-7 cells. a) Structures of the CMV-p40, PGK-p40, CMV-EGFP, and PGK-EGFP constructs. b) Transient EGFP and Il-12 p40 expression in transfected SHK and COS-7. EGFP mean fluorescence intensity (MFI) was measured by flow flow cytometry, and IL-12 p40 expression was assayed by ELISA. Values of EGFP and IL-12 p40 expression shown are the average of three plates transfected in parallel.Back to article page