Figure 1From: Development of patatin knockdown potato tubers using RNA interference (RNAi) technology, for the production of human-therapeutic glycoproteinsMap of patatin-hpRNAi binary vector. A single PCR product (nucleotides 188–798) from the target gene pat3-k1 was cloned into pKANNIBAL vector in an inverted-repeat orientation (sense-oriented copies in XhoI – KpnI site; anti-sense oriented copies in ClaI – XbaI site). pKANNIBAL vector was digested with NotI enzyme and then NotI fragments were cloned into pART27 binary vector.Back to article page