Expression of recombinant Rhipicephalus (Boophilus) microplus, R. annulatus and R. decoloratus Bm86 orthologs as secreted proteins in Pichia pastoris

Table 3 Characterization of the fermentation process for the secretion of recombinant Bm86, Bd86 and Ba86.

Recombinant strain	Mut phenotype	O.D. _{600 nm} before induction	μ_max in glycerol (h^-1)^a	μ_max in methanol (h^-1)^b	Total protein concentration (mg·L^-1)^c	Recombinant protein
						Concentration (mg·L^-1)^c	Purity (%)^c	Productivity (mg·L^-1·h^-1)^c
GS115Moz9-2	Mut^S	115	0.181	0.005	274	150	55	2.1
KM71HDec8-1	Mut^S	125	0.182	0.002	194	110	57	1.5
X33pBaI 3	Mut^S	125	0.178	0.003	170	112	66	1.6

^aThe maximum growth rate (μ_max) was determined during the exponential growth phase on glycerol in batch and fedbatch modes.
^bThe maximum growth rate (μ_max) was determined during the exponential growth phase on methanol (first 20–24 hrs after induction).
^cDetermined in the culture medium 72 hrs after induction with methanol using the Bradford method with BSA as standard [49] and the Experion semiautomated electrophoresis system (Bio-Rad, Hercules, CA, USA).

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