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Figure 8 | BMC Biotechnology

Figure 8

From: Plasmid-based genetic modification of human bone marrow-derived stromal cells: analysis of cell survival and transgene expression after transplantation in rat spinal cord

Figure 8

Tumorigenicity of hMSC-EGFP cell transplants. Left side: histological assessment of tumour growth by hMSC-EGFP cell transplants in rat spinal cord between day 1 and week 2 post-transplantation in immune suppressed animals (+ cyclosporine A). Right side: histological assessment of tumour regression in rat spinal cord at week 4 post-transplantation upon withdrawal of immune suppression at week 2 post-transplantation (-cyclosporine A). First row: hematoxylin-eosin (HE) staining indicating localisation and general appearance of transplantation site. Second row: direct EGFP fluorescence indicating the presence or absence of EGFP positive hMSC-EGFP cell transplants. Third row: immuno-histochemical staining for EGFP indicating the presence or absence of EGFP positive hMSC-EGFP cell transplants. Fourth row: immuno-histochemical staining for CD68 indicating macrophage infiltration into the transplantation site. Representative pictures were chosen from multiple hMSC-EGFP cell transplanted spinal cords analysed 1 day (n = 4), 2 weeks (n = 3) and 4 weeks (n = 2) post-transplantation.

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BMC Biotechnology

ISSN: 1472-6750

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