Figure 11

Stability comparison between EvaGreen (open circle) and SYBR Green I (filled square). EvaGreen (11.42 μM) and SYBR Green I (12.83 μM) were each incubated in 100 mM Tris pH 8.0 at 99°C and sampled at time 0, 0.5, 1 and 3 hours for absorption spectrum measurement at room temperature. ROX was added to each solution to give an absorbance of 0.17 at 580 nm and was used as a stable reference to correct for spectral baseline shift due to either solvent evaporation or instrument instability. The normalized peak intensities of each dye in absorbance at each time points (relative to 0 hour) were plotted against the hours the dye stayed at 99°C.