Figure 3From: Expression of a crown gall biological control phenotype in an avirulent strain of Agrobacterium vitis by addition of the trifolitoxin production and resistance genesEffect of TFX production and inoculum ratio on gall formation. Legend: Nicotiana glauca stems were wounded with a dissecting needle, and 5 ul of inoculum (mixtures noted at left) was placed on the wounds. The top stem was inoculated with CG435 as a positive control. Stems 2 and 3 were inoculated with mixtures of biological control test strains and CG435 at either a 1:1 (left three inoculation sites on each stem) or 10:1 (right three inoculation sites) biological control:pathogen ratios. The photograph was taken approximately 2 months post-inoculation. pT2TFXK confers biological control on F2/5 at the 10:1 ratio, but not at the 1:1 ratio.Back to article page