Table 3 Primer sequences used in this study
From: A single blastocyst assay optimized for detecting CRISPR/Cas9 system-induced indel mutations in mice
Primers | Sequences (5′-3′) | Purpose |
|---|---|---|
3525A | CCGAAGGTAACTGGCTTCAGCAGAGCGCAG | Optimized nuclease treatment (Figure 1) |
bGpA2 | AACATATGCCATATGCTGGCTGCCATGAAC | Optimized nuclease treatment (Figure 1) |
5S | GCCCGAGCTGGAAGCGAGAG | CRISPR/Cas9-mediated mutation (Figure 2A-D) |
6A | AAGTAGGAAGCAGCATTAAGT | CRLSPR/Cas9-mediated mutation (Figure 2A-D) |
1S | CTGAGTTGTGATAGCTGGCA | CRISPR/Cas9-mediated HR (Figure 4A-C) |
3S | AACGTGACCTTAGCCAAGTC | CRISPR/Cas9-niediated HR (Figure 4A-C) |
2A | AGTCCAGTTGCACCAGTCCTTG | CRISPR/Cas9-mediated HR (Figure 4A-C) |
4A | GTACTCATACCAGCAAGGTAG | CRISPR/Cas9-mediated HR (Figure 4A-C) |
PGKL1 | GTTGGCGCCTACCGGTGGATGTGGAATGTG | CRISPR/Cas9-mediated HR (Figure 4A-C) |
PGKL2 | TGTGQGAGGCCAGAGGCCACTIGTGTAG | CRISPR/Cas9-mediated HR (Figure 4A-C) |
RbGA472S | TTCCTCCTCTCCTGACTACTCCCAGTCATA | CRISPR/Cas9-mediated FIR (Figure 4A-C) |
RbGA504S | CTGTCCCTCTTCTCTTATGAAGATCCCTC | CRISPR/Cas9-mediated HR (Figure 4A-C) |