From: Optimized DNA extraction from neonatal dried blood spots: application in methylome profiling
Protocol | DNA quantity (ng)a | 260/280 ratio (after heating)b | 260/230 ratio (after heating)c | DNA integrityd | Detectability by PCR |
---|---|---|---|---|---|
GQ | 808 ± 376 ng | 1.83 ± 0.14 | 0.92 ± 0.23 | Peak intensity > 1 Kbp (25/32) | Detectable (42/42) |
Lower (3/3) 175 ± 73 ng | Similar (2/3); Worse (1/3) | Lower (3/3) | NAe | Similar (3/3) | |
Higher (6/6) by 2.0 × | Worse (6/6) 0.89 ± 0.22 | Similar (6/6) | Similar (4/6); Better (1/6); Worse (1/6) | Similar (6/6) | |
GN or Gn | Higher (4/6) by 1.5 ×; Similar (2/6) | Similar (6/6) | Similar (4/6); Higher (2/6) | Worse (4/4) | Similar (6/6) |
GN-XS or Gn-XS | Higher (6/9) by 1.7 ×; Similar (2/9); Lower (1/9) by 0.4 × | Similar (6/9); Worse (3/9) | Similar (5/9); Lower (4/9) | Worse (6/6) | Similar (9/9) |
NN or Nn | Higher (8/9) by 1.7 ×; Similar (1/9) | Similar (9/9) | Higher (9/9) 1.71 ± 0.20 | Worse (6/6) | Similar (9/9) |
NN-XS | Similar (2/6); Higher (2/6) by 1.8 ×; Lower (2/6) by 0.3 × | Similar (5/6); Worse (1/6) | Similar (6/6) | Worse (3/5); Similar (1/5); Better (1/5) | Similar (6/6) |