Table 1 Primer sequences used for qPCR analysis
From: Characterizing metabolic interactions in a clostridial co-culture for consolidated bioprocessing
Primer name | Target species | Primer sequence (5′-3′) | Product length | Tm |
|---|---|---|---|---|
CA2 (forward) | C. acetobutylicum | CTTGTTGGTGAGGTAACGG | 386 bp | 60°C |
CA2 (reverse) | C. acetobutylicum | CACTCCAGACATCCAGTTTG | ||
CC2 (forward) | C. cellulolyticum | TACAGGGGGATAACACAGG | 348 bp | 60°C |
CC2 (reverse) | C. cellulolyticum | CGTGGCTTATTCTTCAGGTAC | Â | Â |