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Figure 2 | BMC Biotechnology

Figure 2

From: Secretory expression of biologically active human Herpes virus interleukin-10 analogues in Escherichia colivia a modified Sec-dependent transporter construct

Figure 2

Immunoblot analysis of viral IL-10 recombinant proteins. E. coli BL21 (DE3) derived recombinant viral IL-10 proteins were analyzed by immunoblot in different concentrations and from different cell compartments under both reducing (A = HCMV IL-10; C = EBV IL-10) and non-reducing conditions (B = HCMV IL-10). Periplasmic and cytoplasmic fractions of pUC19 transformed E. coli BL21 (DE3) cells and commercial viral IL-10 proteins served as controls. One experiment representative of three is shown. SN = supernatant; PP = periplasmic fraction; CP = cytoplasmic fraction; Com. = commercial; Rec. = recombinant.

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BMC Biotechnology

ISSN: 1472-6750

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