Table 4 List of bacterial strains and plasmids used in this study

Strains

E. coli DH5α

supE44 Δ(lacZYA-argF) U169 (Φ80lacZ ΔM15) hsdR17 recA endA1 gyrA96 thi-1 relA1

Invitrogen

E. coli BW25113

lacI ^q rrnB _T14 Δ lacZ _WJ16 hsdR514 Δ araBAD _AH33 Δ rhaBAD _LD78

42

E. coli BW25113 (Δ gdhA Δ aceE, P_T5-dxs)

E. coli BW25113, Δ gdhA, Δ aceE, replacement of native promoter of the dxs gene with T5 promoter

19

E. coli CBW12241

CIChE strain of E. coli BW25113 (Δ gdhA Δ aceE, P_T5-dxs) resistance to 8 μM triclosan, Δ recA

This study

E. coli CBW12241 (P_T5-appY)

E. coli CBW12241, replacement of native promoter of the appY gene with T5 promoter

This study

E. coli CBW12241 (Δ iclR)

E. coli CBW12241, Δ iclR

This study

E. coli CBW12241 (Δ iclR, P_T5-appY)

E. coli CBW12241(P_T5-appY), Δ iclR

This study

E. coli CBW12251

E. coli CBW12241 (Δ iclR, P_T5-appY) with an additional chromosomal copy of dxs under the control of T5 promoter

Plasmid

pP21KF3T5b

CIChE integration expression vector, Kan^r

29

pP21KF3T5b-crtEBIipi

pP21KF3T5b derivative containing crtE, crtB and crtI gene from Pantoea agglomerans and ipiHPI from Haematococcus pluvialis, Kan^r

This study

pAH121

Helper plasmid expressing phage P21 Int, Amp^r

28

pBAD24-WZM1

pBAD24 derivative containing crtE, crtB and crtI gene from Pantoea agglomerans and ipiHPI from Haematococcus pluvialis, Amp^r

19

pSIM6

pSC101 replicon^ts P_L-gam-bet-exo cI857, Amp^r

43

pKD4

oriRγ, FRT::kan::FRT template plasmid, Kan^r, Amp^r

42

pCP20

pSC101 replicon^ts Flp(λRp) cI857, Cm^r, Amp^r

42

pBAD24

pMB1 ori, P_BAD L-arabinose inducible, Amp^r

41

pBappY

pBAD24 derivative containing the appY gene from E. coli, Amp^r

This study

pBidi

pBAD24 derivative containing the idi gene from E. coli, Amp^r

This study

pBpck

pBAD24 derivative containing the pck gene from E. coli, Amp^r

This study

pBpps

pBAD24 derivative containing the pps gene from E. coli, Amp^r

This study

pBrpoS

pBAD24 derivative containing the rpoS gene from E. coli, Amp^r

This study

pBycgW

pBAD24 derivative containing the ycgW gene from E. coli, Amp^r

This study

pByjiD

pBAD24 derivative containing the yjiD gene from E. coli, Amp^r

This study

pQE30

ColE1 ori, P_T5 IPTG inducible, Amp^r

Qiagen

pQEwrbA

pQE30 derivative containing the wrbA gene from E. coli, Amp^r

This study

pQydeO

pQE30 derivative containing the ydeO gene from E. coli, Amp^r

This study

pQatpE

pQE30 derivative containing the atpE gene from E. coli, Amp^r

This study

pQdxs

pQE30 derivative containing the dxs gene from Bacillus subtilis, Amp^r

This study