Strain or plasmid | Relevant characteristics | References |
---|---|---|
Strains | ||
P. putida KT2440 | Prototrophic, reference strain | http://openwetcare.org/wiki/E._coli_genotypes |
E. coli W3110 | Wild type, xylAB donor | http://openwetcare.org/wiki/E._coli_genotypes |
E. coli JM109 | endA1, glnV44, gyrA96, thi-1, mcrB+, hsdR17 (rk–, mk+), relA1, supE44, [F' traD36 proAB+lacIqlacZΔM15] | http://openwetcare.org/wiki/E._coli_genotypes |
E. coli HB101 | F-, hsdS20 (rB- mB-) recA13, ara-14, proA2, lacY1, galK2, xyl-5, mtl-1, rpsL20 (SmR). | http://openwetcare.org/wiki/E._coli_genotypes |
Plasmids | Â | Â |
RK600 | Cmr, ColE1, oriV, RK2, mob+, tra+ | [38] |
pVLT33 | Kmr, Ptac, MCS of pUCP18, hybrid broad-host-range expression vector | [39] |
pSLM1 | xylA and xylB cloned into pVLT33 | this study |