Production of medium-chain-length polyhydroxyalkanoates by sequential feeding of xylose and octanoic acid in engineered Pseudomonas putida KT2440

Table 2 PHA production in batch and fed-batch fermentations

Entry	Strains	Fermentation type	Carbon source		Feeding type/duration	Feeding phase	Flow	Total amount of fed octanoic acid	OD₆₀₀	μ_max (h^-1)	PHA content (% w w^-1)
			growth substrate	PHA precusor
A	KT2440 (pVLT33)	Batch	xylose	-	-	-	-		no growth	-	-
B	KT2440 (pSLM1)	Batch	xylose	-	-	-	-		0.99	0.24	0.3
C	KT2440 (pSLM1)	Batch	octanoic acid	-	-	-	-	1.44 g L^-1	2.77	0.35	21.0
D	KT2440 (pSLM1)	Fed-Batch	xylose	octanoic acid	linear/12 h	mid exp. ⁽¹⁾	0.072 g L^-1 h^-1	0.87 g L^-1	1.48	0.24	12.1
E	KT2440 (pSLM1)	Fed-Batch	xylose	octanoic acid	linear/8 h	end exp. ⁽²⁾	0.072 g L^-1 h^-1	0.58 g L^-1	1.52	0.25	16.2
F	KT2440 (pSLM1)	Fed-Batch	xylose	octanoic acid	linear/4 h	end exp. ⁽²⁾	0.288 g L^-1 h^-1	1.15 g L^-1	1.4	0.25	20
G	KT2440 (pSLM1)	Batch	xylose + octanoic acid	octanoic acid	-	-	-	1.44 g L^-1	2.13	0.27	28.7

The cells were grown in 0.2NE2 minimal media supplemented with different carbon sources in 1 L mini-bioreactors with a working volume of 800 mL. Samples were taken regularly to measure growth, nitrogen concentration, carbon source present in the medium and PHA contents. Data points are the averages of duplicate measurements.
⁽¹⁾: Feeding started at the middle of exponential batch phase, OD₆₀₀ of 0.5.
⁽²⁾: Feeding started at the end of exponential batch phase, OD₆₀₀ of 1.

ISSN: 1472-6750