Table I Useful PCR primers
From: Mutant loxP vectors for selectable marker recycle and conditional knock-outs
Primer | (vector) | Length | Sequence |
|---|---|---|---|
NE2 | (pMloxNeo) | 30 mer | GGCTGACCGCTTCCTCGTGCTTTACGGTAT |
PU4 | (pMloxPuro) | 29 mer | CAGCGCCCGACCGAAAGGAGCGCACGACC |
BS1 | (pMloxBsr) | 33 mer | CGATTGAAGAACTCATTCCACTCAAATATACCC |
forward | (pExpress) | 30 mer | TTATTGTGCTGTCTCATCATTTTGGCAAAG |
reverse | (pExpress) | 30 mer | CTCTACAAATGTGGTATGGCTGATTATGAT |