Articles

Integrating vectors for genetic studies in the rare Actinomycete Amycolatopsis marina

Few natural product pathways from rare Actinomycetes have been studied due to the difficulty in applying molecular approaches in these genetically intractable organisms. In this study, we sought to identify mo...

Authors: Hong Gao, Buvani Murugesan, Janina Hoßbach, Stephanie K. Evans, W. Marshall Stark and Margaret C. M. Smith

Copy number determination of the gene for the human pancreatic polypeptide receptor NPY4R using read depth analysis and droplet digital PCR

Copy number variation (CNV) plays an important role in human genetic diversity and has been associated with multiple complex disorders. Here we investigate a CNV on chromosome 10q11.22 that spans NPY4R, the gene ...

Authors: Kateryna Shebanits, Torsten Günther, Anna C. V. Johansson, Khurram Maqbool, Lars Feuk, Mattias Jakobsson and Dan Larhammar

A high throughput method for total alcohol determination in fermentation broths

The potassium dichromate oxidation method used in determination of alcohols in fermentation has two major disadvantages. This method cannot be used to determine alcohols in raw fermentation broth samples, whic...

Authors: Peng Zhang, Hao Hai, Dongxu Sun, Weihua Yuan, Weijie Liu, Ruru Ding, Mengting Teng, Lin Ma, Jun Tian and Caifa Chen

A novel autolysis system for extracellular production and direct immobilization of a phospholipase D fused with cellulose binding domain

Several types of phospholipases have been described in phospholipids modification. The majority of phospholipase D (PLD) superfamily members can catalyze two separate reactions: the hydrolysis of phospholipids...

Authors: Haiyang Zhang, Wenqin Chu, Jianan Sun, Zhen Liu, Wen-can Huang, Changhu Xue and Xiangzhao Mao

Novel anti-CD38 humanized mAb SG003 possessed enhanced cytotoxicity in lymphoma than Daratumumab via antibody-dependent cell-mediated cytotoxicity

In vivo use of monoclonal antibodies has become routine clinical practice in the treatment of human cancer. CD38 is an attractive target, because it has double roles, as a receptor and an ectoenzyme. Daratumum...

Authors: Tao Yu, Chunxia Qiao, Ming Lv and Luqun Tang

Recovery of recombinant Mycobacterium tuberculosis antigens fused with cell wall-anchoring motif (LysM) from inclusion bodies using non-denaturing reagent (N-laurylsarcosine)

The current limitations of conventional BCG vaccines highlights the importance in developing novel and effective vaccines against tuberculosis (TB). The utilization of probiotics such as Lactobacillus plantarum f...

Authors: Anhar Danial Mustafa, Jeevanathan Kalyanasundram, Sarah Sabidi, Adelene Ai-Lian Song, Maha Abdullah, Raha Abdul Rahim and Khatijah Yusoff

Surface patches on recombinant erythropoietin predict protein solubility: engineering proteins to minimise aggregation

Protein solubility characteristics are important determinants of success for recombinant proteins in relation to expression, purification, storage and administration. Escherichia coli offers a cost-efficient expr...

Authors: M. Alejandro Carballo-Amador, Edward A. McKenzie, Alan J. Dickson and Jim Warwicker

Frequency of off-targeting in genome edited pigs produced via direct injection of the CRISPR/Cas9 system into developing embryos

The CRISPR/Cas9 system can effectively introduce site-specific modifications to the genome. The efficiency is high enough to induce targeted genome modifications during embryogenesis, thus increasing the effic...

Authors: Kayla Carey, Junghyun Ryu, Kyungjun Uh, Andrea J. Lengi, Sherrie Clark-Deener, Benjamin A. Corl and Kiho Lee

Mutagenesis of FAD2 genes in peanut with CRISPR/Cas9 based gene editing

Increasing the content of oleic acid in peanut seeds is one of the major goals in peanut breeding due to consumer and industry benefits, such as anti-oxidation and long shelf-life. Homeologous ahFAD2A and ahFAD2B

Authors: Mei Yuan, Jun Zhu, Limin Gong, Liangqiong He, Crystal Lee, Suoyi Han, Charles Chen and Guohao He

Efficient gene transfer into T lymphocytes by fiber-modified human adenovirus 5

The gene transduction efficiency of adenovirus to hematopoietic cells, especially T lymphocytes, is needed to be improved. The purpose of this study is to improve the transduction efficiency of T lymphocytes b...

Authors: Yun Lv, Feng-Jun Xiao, Yi Wang, Xiao-Hui Zou, Hua Wang, Hai-Yan Wang, Li-Sheng Wang and Zhuo-Zhuang Lu

Characterisation of three novel α-L-arabinofuranosidases from a compost metagenome

The importance of the accessory enzymes such as α-L-arabinofuranosidases (AFases) in synergistic interactions within cellulolytic mixtures has introduced a paradigm shift in the search for hydrolytic enzymes. ...

Authors: Brent Fortune, Sizwe Mhlongo, Leonardo Joaquim van Zyl, Robert Huddy, Mariette Smart and Marla Trindade

Establishment of an erythroid progenitor cell line capable of enucleation achieved with an inducible c-Myc vector

A robust scalable method for producing enucleated red blood cells (RBCs) is not only a process to produce packed RBC units for transfusion but a potential platform to produce modified RBCs with applications in...

Authors: Steven Mayers, Pablo Diego Moço, Talha Maqbool, Pamuditha N. Silva, Dawn M. Kilkenny and Julie Audet

FlexiBAC: a versatile, open-source baculovirus vector system for protein expression, secretion, and proteolytic processing

Baculovirus-mediated expression in insect cells is a powerful approach for protein production. However, many existing methods are time-consuming, offer limited options for protein tagging, and are unsuitable f...

Authors: Régis P. Lemaitre, Aliona Bogdanova, Barbara Borgonovo, Jeffrey B. Woodruff and David N. Drechsel

Enhancing methane production using anaerobic co-digestion of waste activated sludge with combined fruit waste and cheese whey

Recently, it has been indicated that anaerobic co-digestion of waste activated sludge with other waste streams at wastewater treatment plants is a promising strategy for enhancing methane production and materi...

Authors: Seyed Mostafa Hallaji, Mohammad Kuroshkarim and Seyede Parvin Moussavi

Design of a generic CRISPR-Cas9 approach using the same sgRNA to perform gene editing at distinct loci

The CRISPR/Cas (clustered regularly interspaced short palindromic repeat and CRISPR-associated nucleases) based technologies have revolutionized genome engineering. While their use for prokaryotic genome editi...

Authors: Soumaya Najah, Corinne Saulnier, Jean-Luc Pernodet and Stéphanie Bury-Moné

Inhibition of extracellular proteases improves the production of a xylanase in Parageobacillus thermoglucosidasius

Parageobacillus thermoglucosidasius is a thermophilic and ethanol-producing bacterium capable of utilising both hexose and pentose sugars for fermentation. The organism has been proposed to be a suitable organism...

Authors: Alexandria T. N. Holland, Michael J. Danson and Albert Bolhuis

Formulation and evaluation of norcanthridin nanoemulsions against the Plutella xylostella (Lepidotera: Plutellidae)

Norcantharidin (NCTD), a demethylated derivative of cantharidin (defensive toxin of blister beetles), has been reported to exhibit insecticidal activity against various types of agricultural pests. However, NC...

Authors: Liya Zeng, Yongchang Liu, Jun Pan and Xiaowen Liu

Nicotiana benthamiana is a suitable transient system for high-level expression of an active inhibitor of cotton boll weevil α-amylase

Insect resistance in crops represents a main challenge for agriculture. Transgenic approaches based on proteins displaying insect resistance properties are widely used as efficient breeding strategies. To exte...

Authors: Guilherme Souza Prado, Pingdwende Kader Aziz Bamogo, Joel Antônio Cordeiro de Abreu, François-Xavier Gillet, Vanessa Olinto dos Santos, Maria Cristina Mattar Silva, Jean-Paul Brizard, Marcelo Porto Bemquerer, Martine Bangratz, Christophe Brugidou, Drissa Sérémé, Maria Fatima Grossi-de-Sa and Séverine Lacombe

Ex vivo evolution of human antibodies by CRISPR-X: from a naive B cell repertoire to affinity matured antibodies

Authors: Marie-Claire Devilder, Melinda Moyon, Laetitia Gautreau-Rolland, Benjamin Navet, Jeanne Perroteau, Florent Delbos, Marie-Claude Gesnel, Richard Breathnach and Xavier Saulquin

A new prokaryotic expression vector for the expression of antimicrobial peptide abaecin using SUMO fusion tag

Despite the growing demand for antimicrobial peptides (AMPs) for clinical use as an alternative approach against antibiotic-resistant bacteria, the manufacture of AMPs relies on expensive, small-scale chemical...

Authors: Da Sol Kim, Seon Woong Kim, Jae Min Song, Soon Young Kim and Kwang-Chul Kwon

Correction to: characterizing active transportation mechanisms for free fatty acids and antibiotics in Synechocystis sp. PCC 6803

Following publication of the original article [1], the author reported that the gene/protein names of slr2131 and sll0180 were swapped in the Discussion section. The details of the correction are mentioned below:

Authors: Matthew P. A. Bellefleur, Soo-Young Wanda and Roy Curtiss III

Production of high titer of citric acid from inulin

Citric acid is considered as the most economically feasible product of microbiological production, therefore studies on cheap and renewable raw materials for its production are highly desirable. In this study ...

Authors: Magdalena Rakicka, Jakub Wolniak, Zbigniew Lazar and Waldemar Rymowicz

Resveratrol alleviates lipopolysaccharide-induced inflammation in PC-12 cells and in rat model

Spinal cord injury (SCI) remains a huge medical problem nowadays as there is no hospital providing the versatile strategies for repairing central nervous system and restoring function. Herein, we focused on PC...

Authors: Guiqi Zhang, Yi Liu, Lichen Xu, Chunhe Sha, Haibin Zhang and Weibing Xu

CRISPR-Cas9 mediated targeted disruption of FAD2–2 microsomal omega-6 desaturase in soybean (Glycine max.L)

Recent innovation in the field of genome engineering encompasses numerous levels of plant genome engineering which attract the substantial excitement of plant biologist worldwide. RNA-guided CRISPR Cas9 system...

Authors: Noor al Amin, Naveed Ahmad, Nan Wu, Xiumin Pu, Tong Ma, Yeyao Du, Xiaoxue Bo, Nan Wang, Rahat Sharif and Piwu Wang

Extracellular overproduction of E7 oncoprotein of Iranian human papillomavirus type 16 by genetically engineered Lactococcus lactis

We aimed at constructing Lactococcus lactis strains expressing HPV-16 recombinant E7 (rE7) oncoprotein and examining its overproduction ability followed by optimizing batch and fed-batch fermentations. Thereafter...

Authors: Amir Hossein Mohseni, Sedigheh Taghinezhad-S, Hossein Keyvani and Vadood Razavilar

Homologous recombination-mediated targeted integration in monkey embryos using TALE nucleases

Non-human primate (NHP) models can closely mimic human physiological functions and are therefore highly valuable in biomedical research. Genome editing is now developing rapidly due to the precision and effici...

Authors: Chu Chu, Zhaohui Yang, Jiayin Yang, Li Yan, Chenyang Si, Yu Kang, Zhenzhen Chen, Yongchang Chen, Weizhi Ji and Yuyu Niu

MicroRNA-218 promotes early chondrogenesis of mesenchymal stem cells and inhibits later chondrocyte maturation

MicroRNAs (miRNAs) reportedly participate in the mesenchymal stem cell (MSC) chondrogenic differentiation regulation. We objected to examine how miR-218 regulate chondrogenic differentiation of synovium-derive...

Authors: Song Chen, Zhenyu Xu, Jiahua Shao, Peiliang Fu and Haishan Wu

Characterizing active transportation mechanisms for free fatty acids and antibiotics in Synechocystis sp. PCC 6803

Synechocystis sp. PCC 6803 is a photosynthetic bacterium that has been genetically modified to produce industrially relevant chemicals, yet efflux mechanisms have not been well elucidated. These photosynthetic or...

Authors: Matthew P. A. Bellefleur, Soo-Young Wanda and Roy Curtiss III

Evaluation of strategies for improving the transgene expression in an oleaginous microalga Scenedesmus acutus

Genetic transformation of microalgae has been hampered by inefficient transgene expression, limiting the progress of microalgal biotechnology. Many vector tools and strategies have been developed in recent yea...

Authors: Anongpat Suttangkakul, Anchalee Sirikhachornkit, Piyada Juntawong, Wilasinee Puangtame, Thitikorn Chomtong, Suchada Srifa, Sukhita Sathitnaitham, Wasawat Dumrongthawatchai, Kanidtha Jariyachawalid and Supachai Vuttipongchaikij

An auto-inducible phosphate-controlled expression system of Bacillus licheniformis

A promoter that drives high-level, long-term expression of the target gene under substrate limited growth conditions in the absence of an artificial inducer would facilitate the efficient production of heterol...

Authors: Nguyen Thanh Trung, Nguyen Minh Hung, Nguyen Huy Thuan, Nguyen Xuan Canh, Thomas Schweder and Britta Jürgen

Fast gene disruption in Trichoderma reesei using in vitro assembled Cas9/gRNA complex

CRISPR/Cas9 has wide application potentials in a variety of biological species including Trichoderma reesei, a filamentous fungus workhorse for cellulase production. However, expression of Cas9 heterologously in ...

Authors: Zhenzhen Hao and Xiaoyun Su

Establishment of TP53-knockout canine cells using optimized CRIPSR/Cas9 vector system for canine cancer research

Genetic engineering technology such as clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system provides a powerful tool for developing disease models and determining gene functions. Rece...

Authors: Kiyoung Eun, Min Gi Park, Yeon Woo Jeong, Yeon Ik Jeong, Sang-Hwan Hyun, Woo Suk Hwang, Sung-Hak Kim and Hyunggee Kim

Alterations in the proteomic composition of Serratia marcescens in response to manganese (II)

Proteomics is an important tool for the investigation of dynamic physiological responses of microbes under heavy metal stress. To gain insight into how bacteria respond to manganese (II) and identify the prote...

Authors: Pollyana Santos Queiroz, France Anne Dias Ruas, Natália Rocha Barboza, William de Castro Borges and Renata Guerra-Sá

A chicken bioreactor for efficient production of functional cytokines

The global market for protein drugs has the highest compound annual growth rate of any pharmaceutical class but their availability, especially outside of the US market, is compromised by the high cost of manuf...

Authors: Lissa R. Herron, Clare Pridans, Matthew L. Turnbull, Nikki Smith, Simon Lillico, Adrian Sherman, Hazel J. Gilhooley, Martin Wear, Dominic Kurian, Grigorios Papadakos, Paul Digard, David A. Hume, Andrew C. Gill and Helen M. Sang

Enhancing the efficiency of the Pichia pastoris AOX1 promoter via the synthetic positive feedback circuit of transcription factor Mxr1

The methanol-regulated AOX1 promoter (P_AOX1) is the most widely used promoter in the production of recombinant proteins in the methylotrophic yeast Pichia pastoris. However, as the tight regulation and methanol d...

Authors: Ching-Hsiang Chang, Hao-An Hsiung, Kai-Lin Hong and Ching-Tsan Huang

Recombinant Expression and Bioactivity Comparison of Four Typical Fungal Immunomodulatory Proteins from Three Main Ganoderma Species

More than a dozen of fungal immunomodulatory proteins (FIPs) have been identified to date, most of which are from Ganoderma species. However, little is known about the similarities and differences between differe...

Authors: Zheng-Wei Qu, Si-Ya Zhou, Shi-Xin Guan, Rui Gao, Zuo-Wen Duan, Xin Zhang, Wei-Yan Sun, Wen-Li Fan, Shui-Sen Chen, Li-Jing Chen, Jing-Wei Lin and Yan-Ye Ruan

Simultaneous enzymatic saccharification and comminution for the valorization of lignocellulosic biomass toward natural products

Large-scale processing of lignocellulosics for glucose production generally relies on high temperature and acidic or alkaline conditions. However, extreme conditions produce chemical contaminants that complica...

Authors: Ronald R. Navarro, Yuichiro Otsuka, Masanobu Nojiri, Shigehiro Ishizuka, Masaya Nakamura, Kazuhiro Shikinaka, Kenji Matsuo, Kei Sasaki, Ken Sasaki, Kazuhide Kimbara, Yutaka Nakashimada and Junichi Kato

Association of high pressure and alkaline condition for solubilization of inclusion bodies and refolding of the NS1 protein from zika virus

Proteins in inclusion bodies (IBs) present native-like secondary structures. However, chaotropic agents at denaturing concentrations, which are widely used for IB solubilization and subsequent refolding, unfol...

Authors: Cleide Mara Rosa da Silva, Rosa Maria Chura-Chambi, Lennon Ramos Pereira, Yraima Cordeiro, Luís Carlos de Souza Ferreira and Ligia Morganti

Expression of Rift Valley fever virus N-protein in Nicotiana benthamiana for use as a diagnostic antigen

Rift Valley fever virus (RVFV), the causative agent of Rift Valley fever, is an enveloped single-stranded negative-sense RNA virus in the genus Phlebovirus, family Bunyaviridae. The virus is spread by infected mo...

Authors: Sandiswa Mbewana, Ann E. Meyers, Brandon Weber, Vuyokazi Mareledwane, Maryke L. Ferreira, Phelix A. O. Majiwa and Edward P. Rybicki

High-yield production of human Dicer by transfection of human HEK293-EBNA1 cells grown in suspension

Dicer is a 219-kDa protein that plays key roles in gene regulation, particularly as the ribonuclease III enzyme responsible for cleaving precursor miRNA substrates. Its enzymatic activity is highly regulated b...

Authors: Jonathan Bouvette, Dursun Nizam Korkut, Aurélien Fouillen, Soumiya Amellah, Antonio Nanci, Yves Durocher, James G. Omichinski and Pascale Legault

Myogenic differentiation of primary myoblasts and mesenchymal stromal cells under serum-free conditions on PCL-collagen I-nanoscaffolds

The creation of functional skeletal muscle via tissue engineering holds great promise without sacrificing healthy donor tissue. Different cell types have been investigated regarding their myogenic differentiat...

Authors: Aijia Cai, Moritz Hardt, Paul Schneider, Rafael Schmid, Claudia Lange, Dirk Dippold, Dirk W. Schubert, Anja M. Boos, Annika Weigand, Andreas Arkudas, Raymund E. Horch and Justus P. Beier

Suitability of nutrients removal from brewery wastewater using a hydroponic technology with Typha latifolia

This study aims to assess suitability of hydroponic technology for treatment of brewery wastewater in a hydroponic bioreactor using Typha latifolia. Triplicated hydroponic bioreactor treatment units were designed...

Authors: Abebe Gebeyehu, Nurelegne Shebeshe, Helmut Kloos and Solomon Belay

Development of luciferase-linked antibody capture assay based on luciferase immunoprecipitation systems for antibody detection of porcine reproductive and respiratory syndrome virus

Early detection of porcine reproductive and respiratory syndrome virus (PRRSV) infection of swine is necessary to control this devastating disease. By monitoring host serum antibodies to viral antigens, early ...

Authors: Jie Li, Gang Wang, Di Yang, Bao Zhao, Yongpan Zhao, Yonggang Liu, Xuehui Cai, Yuchen Nan, En-Min Zhou and Chunyan Wu

Cyclodextrin enhanced the soluble expression of Bacillus clarkii γ-CGTase in Escherichia coli

Cyclodextrin glycosyltransferases (CGTases) catalyze the synthesis of cyclodextrins, which are circular α-(1,4)-linked glucans used in many applications in the industries related to food, pharmaceuticals, cosm...

Authors: Lei Wang, Sheng Chen and Jing Wu

Bioprocess optimization for pectinase production using Aspergillus niger in a submerged cultivation system

Pectinase enzymes present a high priced category of microbial enzymes with many potential applications in various food and oil industries and an estimated market share of $ 41.4 billion by 2020.

Authors: Hesham A. El Enshasy, Elsayed Ahmed Elsayed, Noorhamizah Suhaimi, Roslinda Abd Malek and Mona Esawy

Infectivity of adeno-associated virus serotypes in mouse testis

Recombinant adeno-associated viruses (AAVs) are emerging as favoured transgene delivery vectors for both research applications and gene therapy. In this context, a thorough investigation of the potential of va...

Authors: Santhanasabapathy Rajasekaran, Jayashree Thatte, Jayaprakash Periasamy, Alok Javali, Manjunath Jayaram, Dwaipayan Sen, Akshaya Krishnagopal, Giridhara R. Jayandharan and Ramkumar Sambasivan

12th Annual Biotechnology Congress (BAC Girona 2018): abstract collection

Authors:

Efficient production of heat-stable antifungal factor through integrating statistical optimization with a two-stage temperature control strategy in Lysobacter enzymogenes OH11

Heat-stable antifungal factor (HSAF) is a newly identified broad-spectrum antifungal antibiotic from the biocontrol agent Lysobacter enzymogenes and is regarded as a potential biological pesticide, due to its nov...

Authors: Bao Tang, Cheng Sun, Yancun Zhao, Huiyong Xu, Gaoge Xu and Fengquan Liu

Genetic transformation of einkorn (Triticum monococcum L. ssp. monococcum L.), a diploid cultivated wheat species

Domesticated einkorn (Triticum monococcum L.) is one of the oldest cultivated cereal crops in the world. Its small genome size (~ 5.7 GB), low ploidy (2n = 2x = 14, A^mA^m) and high genetic polymorphism make this s...

Authors: Dmitry Miroshnichenko, Danila Ashin, Alexander Pushin and Sergey Dolgov

Bioconversion of duck blood cell: process optimization of hydrolytic conditions and peptide hydrolysate characterization

As the protein-laden by-product, red blood cells (RBCs) from poultry blood is a potential source of protein used as food and feed ingredient. However, RBC was currently underutilized. Therefore, it is an urgen...

Authors: Zhaojun Zheng, Xubiao Wei, Tingting Shang, Yan Huang, Cong Hu and Rijun Zhang