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Microbial biotechnology

This section will consider studies that focus on the genetic manipulation and synthetic/systems biology of bacteria, fungi, yeasts and algae in the context of bioproduction, fermentation, metabolic engineering, biocatalysis, bioremediation and biodegradation.

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  1. Temperature tolerance is an important aspect for commercial scale outdoor cultivation of microalgae and cyanobacteria. While various genes are known to be related to Synechocystis sp. PCC6803's heat shock respons...

    Authors: Ulrich M Tillich, Nick Wolter, Philipp Franke, Ulf Dühring and Marcus Frohme

    Citation: BMC Biotechnology 2014 14:66

    Content type: Research article

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  2. Overexpression of foreign genes in Escherichia coli cells is an efficient means to obtain recombinant proteins. The technique is, however, often hampered by misfolding, degradation, aggregation and formation in i...

    Authors: Ying Wang and Yue-Zhong Li

    Citation: BMC Biotechnology 2014 14:53

    Content type: Research article

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  3. Iron is an essential element in several pathways of microbial metabolism, and therefore low iron toxicity is expected on the usage of Fe nanoparticles (NPs). This study aims to determine the effect of Fe NPs o...

    Authors: George Seghal Kiran, Lipton Anuj Nishanth, Sethu Priyadharshini, Kumar Anitha and Joseph Selvin

    Citation: BMC Biotechnology 2014 14:48

    Content type: Research article

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  4. Lignocellulosic materials are a diverse group of substrates that are generally scarce in nutrients, which compromises the tolerance and fermentation performance of the fermenting organism. The problem is exace...

    Authors: Emma Johansson, Charilaos Xiros and Christer Larsson

    Citation: BMC Biotechnology 2014 14:47

    Content type: Research article

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  5. Kluyveromyces marxianus has recently become a species of interest for ethanol production since it can produce ethanol at high temperature and on a wide variety of substrates. However, the reason why this yeast ca...

    Authors: Jing-juan Liang, Mei-ling Zhang, Meng Ding, Zhi-mao Mai, San-xing Wu, Yue Du and Jia-xun Feng

    Citation: BMC Biotechnology 2014 14:45

    Content type: Research article

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  6. Trans-4-hydroxy-L-proline (trans-Hyp), one of the hydroxyproline (Hyp) isomers, is a useful chiral building block in the production of many pharmaceuticals. Although there are some natural biosynthetic pathways o...

    Authors: Yulan Yi, Huakai Sheng, Zhimin Li and Qin Ye

    Citation: BMC Biotechnology 2014 14:44

    Content type: Research article

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  7. The production of ethyl alcohol by fermentation represents the largest scale application of Saccharomyces cerevisiae in industrial biotechnology. Increased worldwide demand for fuel bioethanol is anticipated over...

    Authors: Marta V Semkiv, Kostyantyn V Dmytruk, Charles A Abbas and Andriy A Sibirny

    Citation: BMC Biotechnology 2014 14:42

    Content type: Research article

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  8. Yeasts tolerant to toxic inhibitors from steam-pretreated lignocellulose with xylose co-fermentation capability represent an appealing approach for 2nd generation ethanol production. Whereas rational engineering,...

    Authors: Justin Smith, Eugéne van Rensburg and Johann F Görgens

    Citation: BMC Biotechnology 2014 14:41

    Content type: Research article

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  9. γ-lactamase is used for the resolution of γ-lactam which is utilized in the synthesizing of abacavir and peramivir. In some cases, enzymatic method is the most utilized method because of its high efficiency an...

    Authors: Jianjun Wang, Junge Zhu, Cong Min and Sheng Wu

    Citation: BMC Biotechnology 2014 14:40

    Content type: Research article

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  10. Thermotoga species are organisms of enormous interest from a biotechnological as well as evolutionary point of view. Genetic modifications of Thermotoga spp. are often desired in order to fully release their mult...

    Authors: Dongmei Han, Hui Xu, Rutika Puranik and Zhaohui Xu

    Citation: BMC Biotechnology 2014 14:39

    Content type: Research article

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  11. Lignocellulosic biomass is highly recalcitrant and various pretreatment techniques are needed to facilitate its effective enzymatic hydrolysis to produce sugars for further conversion to bio-based chemicals. I...

    Authors: John Gräsvik, Sandra Winestrand, Monica Normark, Leif J Jönsson and Jyri-Pekka Mikkola

    Citation: BMC Biotechnology 2014 14:34

    Content type: Research article

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  12. 2-phenylethanl (2-PE) and its derivatives are important chemicals, which are widely used in food materials and fine chemical industries and polymers and it’s also a potentially valuable alcohol for next-genera...

    Authors: Haibo Zhang, Mingle Cao, Xinglin Jiang, Huibin Zou, Cong Wang, Xin Xu and Mo Xian

    Citation: BMC Biotechnology 2014 14:30

    Content type: Research article

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  13. For enzyme production, the costs of solid state fermentation (SSF) techniques were lower and the production higher than submerged cultures. A large number of fungal species was known to grow well on moist subs...

    Authors: Reda M El-Shishtawy, Saleh A Mohamed, Abdullah M Asiri, Abu-bakr M Gomaa, Ibrahim H Ibrahim and Hasan A Al-Talhi

    Citation: BMC Biotechnology 2014 14:29

    Content type: Research article

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  14. Efficient utilization of both glucose and xylose is necessary for a competitive ethanol production from lignocellulosic materials. Although many advances have been made in the development of xylose-fermenting ...

    Authors: Basti Bergdahl, Marie F Gorwa-Grauslund and Ed WJ van Niel

    Citation: BMC Biotechnology 2014 14:28

    Content type: Research article

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  15. Inhibitors are formed that reduce the fermentation performance of fermenting yeast during the pretreatment process of lignocellulosic biomass. An exometabolomics approach was applied to systematically identify...

    Authors: Ying Zha, Johan A Westerhuis, Bas Muilwijk, Karin M Overkamp, Bernadien M Nijmeijer, Leon Coulier, Age K Smilde and Peter J Punt

    Citation: BMC Biotechnology 2014 14:22

    Content type: Research article

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  16. Enhancement of enzymatic digestibility by some supplementations could reduce enzyme loading and cost, which is still too high to realize economical production of lignocellulosic biofuels. A recent study indica...

    Authors: Yong Tang, Fuhou Lei, Carrasco Cristhian, Zuguang Liu, Hailong Yu and Jianxin Jiang

    Citation: BMC Biotechnology 2014 14:21

    Content type: Research article

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  17. Insertion duplication mutagenesis (IDM) and in-frame deletion (IFD) are common techniques for studying gene function, and have been applied to pneumolysin (ply), a virulence gene in Streptococcus pneumoniae (D39)...

    Authors: Esther Yip-Mei Liu, Feng-Yee Chang, Jen-Chang Chang and Chang-Phone Fung

    Citation: BMC Biotechnology 2014 14:16

    Content type: Methodology article

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  18. Carbon sources for biofuel production are wide-ranging and their availability depends on the climate and soil conditions of the land where the production chain is located. Henequen (Agave fourcroydes Lem.) is cul...

    Authors: Pablo A Villegas-Silva, Tanit Toledano-Thompson, Blondy B Canto-Canché, Alfonso Larqué-Saavedra and Luis F Barahona-Pérez

    Citation: BMC Biotechnology 2014 14:14

    Content type: Research article

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  19. Efficiently utilizing all available carbon from lignocellulosic feedstock presents a major barrier to the production of economically feasible biofuel. Previously, to enable xylose utilization, we introduced a ...

    Authors: Jin Hou, Fan Suo, Chengqiang Wang, Xiaowei Li, Yu Shen and Xiaoming Bao

    Citation: BMC Biotechnology 2014 14:13

    Content type: Research article

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  20. To connect gene expression with cellular physiology, we need to follow levels of proteins over time. Experiments typically use variants of Green Fluorescent Protein (GFP), and time-series measurements require ...

    Authors: Catherine A Lichten, Rachel White, Ivan BN Clark and Peter S Swain

    Citation: BMC Biotechnology 2014 14:11

    Content type: Methodology article

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  21. Penicillin G acylase (PGA) is used industrially to catalyze the hydrolysis of penicillin G to obtain 6-aminopenicillanic acid. In Escherichia coli, the most-studied microorganism for PGA production, this enzyme a...

    Authors: Ana Maria Vélez, Adilson José da Silva, Antonio Carlos Luperni Horta, Cintia Regina Sargo, Gilson Campani, Gabriel Gonçalves Silva, Raquel de Lima Camargo Giordano and Teresa Cristina Zangirolami

    Citation: BMC Biotechnology 2014 14:6

    Content type: Research article

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  22. Eukaryotic ubiquitin and SUMO are frequently used as tags to enhance the fusion protein expression in microbial host. They increase the solubility and stability, and protect the peptides from proteolytic degra...

    Authors: Sujuan Yuan, Xin Wang, Jian Xu, Zheng Yan and Nan Wang

    Citation: BMC Biotechnology 2014 14:5

    Content type: Research article

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  23. Signalling proteins often contain several well defined and conserved protein domains. Structural analyses of such domains by nuclear magnetic spectroscopy or X-ray crystallography may greatly inform the functi...

    Authors: Thorny Cecilie Bie Andersen, Kjersti Lindsjø, Cecilie Dahl Hem, Lise Koll, Per Eugen Kristiansen, Lars Skjeldal, Amy H Andreotti and Anne Spurkland

    Citation: BMC Biotechnology 2014 14:3

    Content type: Research article

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  24. Streptococcus pneumoniae (S. pneumoniae) causes several serious diseases including pneumonia, septicemia and meningitis. The World Health Organization estimates that streptococcal pneumonia is the cause of approx...

    Authors: Guillermo Marini, Mateus Dalcin Luchese, Ana Paula Correa Argondizzo, Ana Carolina Magalhães Andrade de Góes, Ricardo Galler, Tito Lívio Moitinho Alves, Marco Alberto Medeiros and Ariane Leites Larentis

    Citation: BMC Biotechnology 2014 14:1

    Content type: Research article

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  25. Gas vesicles are hollow, buoyant organelles bounded by a thin and extremely stable protein membrane. They are coded by a cluster of gvp genes in the halophilic archaeon, Halobacterium sp. NRC-1. Using an expressi...

    Authors: Shiladitya DasSarma, Ram Karan, Priya DasSarma, Susan Barnes, Folasade Ekulona and Barbara Smith

    Citation: BMC Biotechnology 2013 13:112

    Content type: Research article

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  26. It remains a challenge for recombinant S. cerevisiae to convert xylose in lignocellulosic biomass hydrolysates to ethanol. Although industrial diploid strains are more robust compared to laboratory haploid strain...

    Authors: Liuyang Diao, Yingmiao Liu, Fenghui Qian, Junjie Yang, Yu Jiang and Sheng Yang

    Citation: BMC Biotechnology 2013 13:110

    Content type: Research article

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  27. Staphylococcus aureus produces several toxins, including Panton-Valentine leukocidin (PVL). The involvement of PVL in primary skin infections, necrotizing pneumonia, musculoskeletal disorders, brain abscess, and ...

    Authors: Charles Emeka Okolie, Alan Cockayne, Christopher Penfold and Richard James

    Citation: BMC Biotechnology 2013 13:103

    Content type: Methodology article

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  28. The complete degradation of the cellulose requires the synergistic action of endo-β-glucanase, exo-β-glucanase, and β-glucosidase. But endo-β-glucanase and exo-β-glucanase can be recovered by solid–liquid sepa...

    Authors: Linguo Zhao, Qian Pang, Jingcong Xie, Jianjun Pei, Fei Wang and Song Fan

    Citation: BMC Biotechnology 2013 13:101

    Content type: Research article

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  29. There is an imperative necessity for alternative sources of energy able to reduce the world dependence of fossil oil. One of the most successful options is ethanol obtained mainly from sugarcane and corn ferme...

    Authors: Diogo Robl, Priscila da Silva Delabona, Carla Montanari Mergel, Juan Diego Rojas, Patrícia dos Santos Costa, Ida Chapaval Pimentel, Vania Aparecida Vicente, José Geraldo da Cruz Pradella and Gabriel Padilla

    Citation: BMC Biotechnology 2013 13:94

    Content type: Research article

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  30. Genome scale metabolic reconstructions are developed to efficiently engineer biocatalysts and bioprocesses based on a rational approach. However, in most reconstructions, due to the lack of appropriate measure...

    Authors: Jozef BJH van Duuren, Jacek Puchałka, Astrid E Mars, René Bücker, Gerrit Eggink, Christoph Wittmann and Vítor AP Martins dos Santos

    Citation: BMC Biotechnology 2013 13:93

    Content type: Research article

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  31. Carboxyl-terminal protease (CtpA) plays essential functions in posttranslational protein processing in prokaryotic and eukaryotic cells. To date, only a few bacterial ctpA genes have been characterized. Here we c...

    Authors: Yunxia Li, Yingjie Pan, Qunxin She and Lanming Chen

    Citation: BMC Biotechnology 2013 13:89

    Content type: Research article

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  32. Xanthophyllomyces dendrorhous is a basidiomycetous yeast that is relevant to biotechnology, as it can synthesize the carotenoid astaxanthin. However, the astaxanthin levels produced by wild-type strains are low. ...

    Authors: Gabriela Contreras, Salvador Barahona, María Cecilia Rojas, Marcelo Baeza, Víctor Cifuentes and Jennifer Alcaíno

    Citation: BMC Biotechnology 2013 13:84

    Content type: Research article

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  33. Interleukin-10 homologues encoded by Herpes viruses such as Epstein-Barr virus (EBV) and human cytomegalovirus (HCMV) hold interesting structural and biological characteristics compared to human interleukin-10...

    Authors: Sarah Förster, Manuela Brandt, Dorothea S Mottok, Anke Zschüttig, Kurt Zimmermann, Frederick R Blattner, Florian Gunzer and Christoph Pöhlmann

    Citation: BMC Biotechnology 2013 13:82

    Content type: Research article

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  34. Anthrax is a zoonotic disease recognized to affect herbivores since Biblical times and has the widest range of susceptible host species of any known pathogen. The ease with which the bacterium can be weaponize...

    Authors: Roland J Saldanha, Adin Pemberton, Patrick Shiflett, Jiri Perutka, Jacob T Whitt, Andrew Ellington, Alan M Lambowitz, Ryan Kramer, Deborah Taylor and Thomas J Lamkin

    Citation: BMC Biotechnology 2013 13:72

    Content type: Methodology article

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  35. As a strong fermentator, Saccharomyces cerevisiae has the potential to be an excellent host for ethanol production by consolidated bioprocessing. For this purpose, it is necessary to transform cellulose genes int...

    Authors: Tzi-Yuan Wang, Chih-Jen Huang, Hsin-Liang Chen, Po-Chun Ho, Huei-Mien Ke, Hsing-Yi Cho, Sz-Kai Ruan, Kuo-Yen Hung, I-Li Wang, Ya-Wun Cai, Huang-Mo Sung, Wen-Hsiung Li and Ming-Che Shih

    Citation: BMC Biotechnology 2013 13:71

    Content type: Research article

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  36. Escherichia coli is most widely used prokaryotic expression system for the production of recombinant proteins. Several strategies have been employed for expressing recombinant proteins in E.coli. This includes th...

    Authors: Narendrakumar Sekar, Soumya Kariyadan Veetil and Muniasamy Neerathilingam

    Citation: BMC Biotechnology 2013 13:70

    Content type: Research article

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  37. The yeast Saccharomyces cerevisiae can be a useful model for studying cellular mechanisms related to sterol synthesis in humans due to the high similarity of the mevalonate pathway between these organisms. This m...

    Authors: Agata Maciejak, Agata Leszczynska, Ilona Warchol, Monika Gora, Joanna Kaminska, Danuta Plochocka, Monika Wysocka-Kapcinska, Dorota Tulacz, Joanna Siedlecka, Ewa Swiezewska, Maciej Sojka, Witold Danikiewicz, Norbert Odolczyk, Anna Szkopinska, Grazyna Sygitowicz and Beata Burzynska

    Citation: BMC Biotechnology 2013 13:68

    Content type: Research article

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  38. Bioflocculants effect the aggregation of suspended solutes in solutions thus, a viable alternative to inorganic poly-ionic and synthetic organic flocculants which are associated with deleterious health problem...

    Authors: Ntsaluba Luvuyo, Uchechukwu U Nwodo, Leonard V Mabinya and Anthony I Okoh

    Citation: BMC Biotechnology 2013 13:62

    Content type: Research article

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  39. The utilization of raw biomass components such as cellulose or hemicellulose for the production of valuable chemicals has attracted considerable research interest in recent years. One promising approach is the...

    Authors: Elena Geiser, Nick Wierckx, Martin Zimmermann and Lars M Blank

    Citation: BMC Biotechnology 2013 13:59

    Content type: Research article

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  40. L-ornithine is effective in the treatment of liver diseases and helps strengthen the heart. The commercial applications mean that efficient biotechnological production of L-ornithine has become increasingly ne...

    Authors: Ling-Yan Jiang, Shang-Guang Chen, Yuan-Yuan Zhang and Jian-Zhong Liu

    Citation: BMC Biotechnology 2013 13:47

    Content type: Research article

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  41. Plant expansins and fungal swollenin that can disrupt crystalline cellulose have great potential for applications in conversion of biomass. Recent studies have been mainly focused on Trichoderma reesei swollenin ...

    Authors: Kang Kang, Shaowen Wang, Guohong Lai, Gang Liu and Miao Xing

    Citation: BMC Biotechnology 2013 13:42

    Content type: Research article

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  42. Biofilms occur on a wide variety of surfaces including metals, ceramics, glass etc. and often leads to accumulation of large number of various microorganisms on the surfaces. This biofilm growth is highly unde...

    Authors: Byung-Doo Lee, Rajagopalan Thiruvengadathan, Sachidevi Puttaswamy, Brandon M Smith, Keshab Gangopadhyay, Shubhra Gangopadhyay and Shramik Sengupta

    Citation: BMC Biotechnology 2013 13:30

    Content type: Research article

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  43. There is a continuous demanding for tightly regulated prokaryotic expression systems, which allow functional synthesis of toxic proteins in Escherichia coli for bioscience or biotechnology application. However, m...

    Authors: Lingyu Guan, Qin Liu, Chao Li and Yuanxing Zhang

    Citation: BMC Biotechnology 2013 13:25

    Content type: Research article

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  44. Tyrosinase is a bifunctional enzyme that catalyzes both the hydroxylation of monophenols to o-diphenols (monophenolase activity) and the subsequent oxidation of the diphenols to o-quinones (diphenolase activity)....

    Authors: Qun Ren, Bernhard Henes, Michael Fairhead and Linda Thöny-Meyer

    Citation: BMC Biotechnology 2013 13:18

    Content type: Research article

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  45. Bioinformatic analysis of the genes coding for the chitinase in Pyrococcus furiosus and Thermococcus kodakarensis revealed that most likely a one nucleotide insertion in Pyrococcus caused a frame shift in the chi...

    Authors: Martina Kreuzer, Karolin Schmutzler, Ingrid Waege, Michael Thomm and Winfried Hausner

    Citation: BMC Biotechnology 2013 13:9

    Content type: Research article

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  46. In recent years, constraint-based metabolic models have emerged as an important tool for metabolic engineering; a number of computational algorithms have been developed for identifying metabolic engineering st...

    Authors: Kai Zhuang, Laurence Yang, William R Cluett and Radhakrishnan Mahadevan

    Citation: BMC Biotechnology 2013 13:8

    Content type: Research article

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