Applied immunology

Colorectal cysts as a validating tool for CAR therapy

Treatment of cancers has largely benefited from the development of immunotherapy. In particular, Chimeric Antigen Receptor (CAR) redirected T cells have demonstrated impressive efficacy against B-cell malignan...

Authors: Pierre Dillard, Maren Lie, Elizabeth Baken, Viola Hélène Lobert, Emmanuelle Benard, Hakan Köksal, Else Marit Inderberg and Sébastien Wälchli

Recombinant PAL/PilE/FlaA DNA vaccine provides protective immunity against Legionella pneumophila in BALB/c mice

Legionella pneumophila (L.pneumophila), a Gram-negative small microorganism, causes hospital-acquired pneumonia especially in immunocompromised patients. Vaccination may be an effective method for preventing L.pn...

Authors: Yingying Chen, Zehui Yang, Ying Dong and Yu Chen

A self-aggregating peptide: implications for the development of thermostable vaccine candidates

The use of biomaterials has been expanded to improve the characteristics of vaccines. Recently we have identified that the peptide PH_(1–110) from polyhedrin self-aggregates and incorporates foreign proteins to fo...

Authors: Adolfo Cruz-Reséndiz, Jesús Zepeda-Cervantes, Alicia Sampieri, Carlos Bastián-Eugenio, Gonzalo Acero, J. Iván Sánchez-Betancourt, Goar Gevorkian and Luis Vaca

Development and applications of a monoclonal antibody against caprine interferon-gamma

Interferon-gamma (IFN-γ) is an important mediator of type I immune response and has antiviral, immunoregulatory and anti-tumor properties, plays a wide range of roles in inflammation and autoimmune diseases. T...

Authors: Wen-Tao Ma, Qi Liu, Meng-Xia Ning, Yu-Xu Qi, Saad Rehman and De-Kun Chen

Tuning the performance of CAR T cell immunotherapies

Simultaneous advances in gene editing, T cell engineering and biotechnology currently provide an opportunity for rapid progress in medicine. The approval of chimeric antigen receptor (CAR) T cell therapies by ...

Authors: Noah H. Richardson, Jordan B. Luttrell, Jonathan S. Bryant, Damian Chamberlain, Saleem Khawaja, Indira Neeli and Marko Radic

Production and characterization of virus-like particles of grapevine fanleaf virus presenting L2 epitope of human papillomavirus minor capsid protein

Virus-like particle (VLP) platform represents a promising approach for the generation of efficient and immunogenic subunit vaccines. Here, the feasibility of using grapevine fanleaf virus (GFLV) VLPs as a new ...

Authors: Razieh Yazdani, Masoud Shams-Bakhsh, Afshin Hassani-Mehraban, Seyed Shahriar Arab, Nicolas Thelen, Marc Thiry, Jacques Crommen, Marianne Fillet, Nathalie Jacobs, Alain Brans and Anne-Catherine Servais

A simple method for in vitro preparation of natural killer cells from cord blood

Cord Blood (CB) has been considered a promising source of natural killer (NK) cells for cellular immunotherapy. However, it is difficult to expand the large numbers of highly pure NK cells from CB without cell...

Authors: Yong Xu Mu, Yu Xia Zhao, Bing Yao Li, Hong Jing Bao, Hui Jiang, Xiao Lei Qi, Li Yun Bai, Yun Hong Wang, Zhi Jie Ma and Xiao Yun Wu

Development of a novel human phage display-derived anti-LAG3 scFv antibody targeting CD8⁺ T lymphocyte exhaustion

Lymphocyte-activation gene (LAG)3 is a 498 aa transmembrane type I protein acting as an immune inhibitory receptor. It is expressed on activated lymphocytes, natural killer cells and plasmacytoid dendritic cel...

Authors: Alessandro Ascione, Claudia Arenaccio, Alessandra Mallano, Michela Flego, Mara Gellini, Mauro Andreotti, Craig Fenwick, Giuseppe Pantaleo, Stefano Vella and Maurizio Federico

Intracellular human antibody fragments recognizing the VP35 protein of Zaire Ebola filovirus inhibit the protein activity

Ebola hemorrhagic fever is caused by the Ebola filovirus (EBOV), which is one of the most aggressive infectious agents known worldwide. The EBOV pathogenesis starts with uncontrolled viral replication and subv...

Authors: Michela Flego, Aldo Frau, Luisa Accardi, Alessandra Mallano, Alessandro Ascione, Mara Gellini, Elisa Fanunza, Stefano Vella, Paola Di Bonito and Enzo Tramontano

Preparation of a novel monoclonal antibody against caprine interleukin-17A and its applications in immunofluorescence and immunohistochemistry assays

Interleukin-17 (IL-17), the characteristic cytokine secreted by T helper 17 lymphocytes (Th17 cells), plays a pivotal role in host defense and many inflammatory or autoimmune diseases. The aim of this study wa...

Authors: Yang Gao, Feng Feng Sang, De Lan Meng, Yi Wang, Wen Tao Ma and De Kun Chen

Ex vivo evolution of human antibodies by CRISPR-X: from a naive B cell repertoire to affinity matured antibodies

Authors: Marie-Claire Devilder, Melinda Moyon, Laetitia Gautreau-Rolland, Benjamin Navet, Jeanne Perroteau, Florent Delbos, Marie-Claude Gesnel, Richard Breathnach and Xavier Saulquin

Membrane-bound and soluble forms of an NMDA receptor extracellular domain retain epitopes targeted in auto-immune encephalitis

Anti-NMDA receptor encephalitis (ANRE) is a potentially lethal disease attributed to auto-antibodies against the N-methyl-D-aspartate receptor (NMDAR). Full recovery is possible if therapy is initiated early i...

Authors: Rashmi Sharma, Fetweh H. Al-Saleem, Rama Devudu Puligedda, Amy Rattelle, David R. Lynch and Scott K. Dessain

Preparation of an epitope-based recombinant diagnostic antigen specific to anti-phospholipase A₂ receptor 1 antibodies

According to recent studies, the phospholipase A2 receptor 1 (PLA₂R1) may be used as a biomarker to diagnose idiopathic membranous nephropathy (iMN). Moreover, the immune-dominant regions of PLA₂R1 have been iden...

Authors: Hua Li, Yonghua Xu, Weiming Yang and Xiaohong Huang

Simultaneous detection of α-Lactoalbumin, β-Lactoglobulin and Lactoferrin in milk by Visualized Microarray

α-Lactalbumin (a-LA), β-lactoglobulin (β-LG) and lactoferrin (LF) are of high nutritional value which have made ingredients of choice in the formulation of modern foods and beverages. There remains an urgent n...

Authors: Zhoumin Li, Fang Wen, Zhonghui Li, Nan Zheng, Jindou Jiang and Danke Xu

Overview of methodologies for T-cell receptor repertoire analysis

The T-cell receptor (TCR), located on the surface of T cells, is responsible for the recognition of the antigen-major histocompatibility complex, leading to the initiation of an inflammatory response. Analysin...

Authors: Elisa Rosati, C Marie Dowds, Evaggelia Liaskou, Eva Kristine Klemsdal Henriksen, Tom H Karlsen and Andre Franke

Evaluation of Gaussia luciferase and foot-and-mouth disease virus 2A translational interrupter chimeras as polycistronic reporters for transgene expression

The Gaussia princeps luciferase is used as a stand-alone reporter of transgene expression for in vitro and in vivo expression systems due to the rapid and easy monitoring of luciferase activity. We sought to simu...

Authors: Michael Puckette, Thomas Burrage, John G. Neilan and Max Rasmussen

A fast and effective determination of the biodistribution and subcellular localization of fluorescent immunoliposomes in freshly excised animal organs

Preclinical research implementing fluorescence-based approaches is inevitable for drug discovery and technology. For example, a variety of contrast agents developed for biomedical imaging are usually evaluated...

Authors: Felista L. Tansi, Ronny Rüger, Ansgar M. Kollmeier, Claudia Böhm, Roland E. Kontermann, Ulf K. Teichgraeber, Alfred Fahr and Ingrid Hilger

Antigen-specific single B cell sorting and expression-cloning from immunoglobulin humanized rats: a rapid and versatile method for the generation of high affinity and discriminative human monoclonal antibodies

There is an ever-increasing need of monoclonal antibodies (mAbs) for biomedical applications and fully human binders are particularly desirable due to their reduced immunogenicity in patients. We have applied ...

Authors: Laure-Hélène Ouisse, Laetitia Gautreau-Rolland, Marie-Claire Devilder, Michael Osborn, Melinda Moyon, Jonathan Visentin, Frank Halary, Marianne Bruggemann, Roland Buelow, Ignacio Anegon and Xavier Saulquin

Novel prokaryotic expression of thioredoxin-fused insulinoma associated protein tyrosine phosphatase 2 (IA-2), its characterization and immunodiagnostic application

The insulinoma associated protein tyrosine phosphatase 2 (IA-2) is one of the immunodominant autoantigens involved in the autoimmune attack to the beta-cell in Type 1 Diabetes Mellitus. In this work we have de...

Authors: Luciano Lucas Guerra, Natalia Inés Faccinetti, Aldana Trabucchi, Bruno David Rovitto, Adriana Victoria Sabljic, Edgardo Poskus, Ruben Francisco Iacono and Silvina Noemí Valdez

Generation of monoclonal antibodies against native viral proteins using antigen-expressing mammalian cells for mouse immunization

Due to their rising incidence and progressive geographical spread, infections with mosquito-borne viruses, such as dengue (DENV), chikungunya and zika virus, have developed into major public health challenges....

Authors: Natalie Rose, Carlos Augusto Pinho-Nascimento, Alessia Ruggieri, Paola Favuzza, Marco Tamborrini, Hanna Roth, Marcia Terezinha Baroni de Moraes, Hugues Matile, Thomas Jänisch, Gerd Pluschke and Katharina Röltgen

New strategy for testing efficacy of immunotherapeutic compounds for diabetes in vitro

The valuable role of immunotherapy in treating autoimmune diseases is increasingly recognized by those involved in the research and clinical application of new biopharmaceuticals products. However, many aspect...

Authors: Gecilmara Salviato Pileggi, Aline Dayana Clemencio, Thiago Malardo, Sonir R Antonini, Vania Luiza Deperon Bonato, Wendy Martin Rios and Celio L Silva

Production of antigen-specific human IgGs by in vitro immunization

We previously developed in vitro immunization based on a fusion protein containing the transcriptional transactivator (Tat) of human immunodeficiency virus and a double domain, called ZZ, derived from protein A o...

Authors: A. Wijkhuisen, A. Savatier, N. Cordeiro and M. Léonetti

Novel fusion proteins for the antigen-specific staining and elimination of B cell receptor-positive cell populations demonstrated by a tetanus toxoid fragment C (TTC) model antigen

In an earlier study we developed a unique strategy allowing us to specifically eliminate antigen-specific murine B cells via their distinct B cell receptors using a new class of fusion proteins. In the present...

Authors: Diana Klose, Ute Saunders, Stefan Barth, Rainer Fischer, Annett Marita Jacobi and Thomas Nachreiner

HybriFree: a robust and rapid method for the development of monoclonal antibodies from different host species

The production of recombinant monoclonal antibodies in mammalian cell culture is of high priority in research and medical fields. A critical step in this process is the isolation of the antigen-binding domain ...

Authors: Gaily Kivi, Kaupo Teesalu, Jüri Parik, Elen Kontkar, Mart Ustav Jr, Liis Noodla, Mart Ustav and Andres Männik

Novel avian single-chain fragment variable (scFv) targets dietary gluten and related natural grain prolamins, toxic entities of celiac disease

Celiac disease (CD) is a chronic, small intestinal inflammatory disease mediated by dietary gluten and related prolamins. The only current therapeutic option is maintenance of a strict life-long gluten-free di...

Authors: Valerie Stadlmann, Hanna Harant, Irina Korschineck, Marcela Hermann, Florian Forster and Albert Missbichler

Defining the complementarities between antibodies and haptens to refine our understanding and aid the prediction of a successful binding interaction

Low molecular weight haptens (<1000 Da) cannot be recognized by the immune system unless conjugated to larger carrier molecules. Antibodies to these exceptionally small antigens can still be generated with exq...

Authors: Mohammed M. Al Qaraghuli, Soumya Palliyil, Gillian Broadbent, David C. Cullen, Keith A. Charlton and Andrew J. Porter

Isolation of nanomolar scFvs of non-human primate origin, cross-neutralizing botulinum neurotoxins A₁ and A₂ by targeting their heavy chain

Botulism is a naturally occurring disease, mainly caused by the ingestion of food contaminated by the botulinum neurotoxins (BoNTs). Botulinum neurotoxins are the most lethal. They are classified among the six...

Authors: Arnaud Avril, Sebastian Miethe, Michel R. Popoff, Christelle Mazuet, Siham Chahboun, Christine Rasetti-Escargueil, Dorothea Sesardic, Philippe Thullier, Michael Hust and Thibaut Pelat

Construction of polyomavirus-derived pseudotype virus-like particles displaying a functionally active neutralizing antibody against hepatitis B virus surface antigen

Virus-like particles (VLPs) can be efficiently produced by heterologous expression of viral structural proteins in yeast. Due to their repetitive structure, VLPs are extensively used for protein engineering an...

Authors: Milda Pleckaityte, Corinna M. Bremer, Alma Gedvilaite, Indre Kucinskaite-Kodze, Dieter Glebe and Aurelija Zvirbliene

Feasibility of Cowpea chlorotic mottle virus-like particles as scaffold for epitope presentations

Within the last decade Virus-Like Particles (VLPs) have increasingly received attention from scientists for their use as a carrier of (peptide) molecules or as scaffold to present epitopes for use in subunit v...

Authors: Afshin Hassani-Mehraban, Sjoerd Creutzburg, Luc van Heereveld and Richard Kormelink

An avian influenza A (H7N9) virus vaccine candidate based on the fusion protein of hemagglutinin globular head and Salmonella typhimurium flagellin

A novel influenza virus, subtype H7N9, circulated through China in 2013–2014. Its higher rates of human infection in a wide range of locations within China and the associated increased likelihood of human-to-h...

Authors: Li Song, Dan Xiong, Xilong Kang, Yun Yang, Jing Wang, Yaxin Guo, Hui Xu, Sujuan Chen, Daxin Peng, Zhiming Pan and Xinan Jiao

Hybrid flagellin as a T cell independent vaccine scaffold

To extend the potency of vaccines against infectious diseases, vaccines should be able to exploit multiple arms of the immune system. One component of the immune system that is under-used in vaccine design is ...

Authors: Kaila M. Bennett, Ronald D. Gorham Jr, Veronica Gusti, Lien Trinh, Dimitrios Morikis and David D. Lo

Generation in yeast of recombinant virus-like particles of porcine circovirus type 2 capsid protein and their use for a serologic assay and development of monoclonal antibodies

Porcine circovirus type 2 (PCV2) is considered to be an important emerging pathogen associated with a number of different syndromes and diseases in pigs known as PCV2-associated diseases. It has been responsib...

Authors: Juozas Nainys, Rita Lasickiene, Rasa Petraityte-Burneikiene, Jonas Dabrisius, Raimundas Lelesius, Vilimas Sereika, Aurelija Zvirbliene, Kestutis Sasnauskas and Alma Gedvilaite

Identification of an antibody fragment specific for androgen-dependent prostate cancer cells

Prostate cancer is the most-diagnosed non-skin cancer among males in the US, and the second leading cause of cancer-related death. Current methods of treatment and diagnosis are not specific for the disease. T...

Authors: Ryan M Williams, Cyrus J Hajiran, Sara Nayeem and Letha J Sooter

Lactococcus lactiscarrying the pValac DNA expression vector coding for IL-10 reduces inflammation in a murine model of experimental colitis

Inflammatory bowel diseases (IBD) are intestinal disorders characterized by inflammation in the gastrointestinal tract. Interleukin-10 is one of the most important anti-inflammatory cytokines involved in the i...

Authors: Meritxell Zurita-Turk, Silvina del Carmen, Ana CG Santos, Vanessa B Pereira, Denise C Cara, Sophie Y Leclercq, Alejandra dM de LeBlanc, Vasco Azevedo, Jean-Marc Chatel, Jean G LeBlanc and Anderson Miyoshi

Novel human recombinant antibodies against Mycobacterium tuberculosis antigen 85B

Tuberculosis is the leading cause of death due to bacterial infections worldwide, mainly caused by Mycobacterium tuberculosis. The antigen 85 complex comprises a set of major secreted proteins of M. tuberculosis,...

Authors: Manon Fuchs, Susanne Kämpfer, Saskia Helmsing, Ralf Spallek, Wulf Oehlmann, Wiebke Prilop, Ronald Frank, Stefan Dübel, Mahavir Singh and Michael Hust

Optimization of heterologous protein production in Chinese hamster ovary cells under overexpression of spliced form of human X-box binding protein

The optimization of protein production is a complex and challenging problem in biotechnology. Different techniques for transcription, translation engineering and the optimization of cell culture conditions hav...

Authors: Galina Gulis, Kelly Cristina Rodrigues Simi, Renata Rodrigues de Toledo, Andrea Queiroz Maranhao and Marcelo Macedo Brigido

Pharmacological characteristics and efficacy of a novel anti-angiogenic antibody FD006 in corneal neovascularization

Vascular endothelial growth factor (VEGF) is a key angiogenic factors. It plays an important role in both physiologic and pathologic angiogenesis and increases permeability across the vessels. Using antibody p...

Authors: Qun Wang, Jing Yang, Kun Tang, Longlong Luo, Liqiang Wang, Lei Tian, Yanming Jiang, Jiannan Feng, Yan Li, Beifen Shen, Ming Lv and Yifei Huang

Simultaneous display of two large proteins on the head and tail of bacteriophage lambda

Consistent progress in the development of bacteriophage lambda display platform as an alternative to filamentous phage display system was achieved in the recent years. The lambda phage has been engineered to d...

Authors: Emiliano Pavoni, Paola Vaccaro, Valeria D’Alessio, Rita De Santis and Olga Minenkova

Mimotope vaccination for epitope-specific induction of anti-VEGF antibodies

Tumor angiogenesis is critical for tumor growth, infiltration and metastasis. Vascular endothelial growth factor (VEGF) is a potent angiogenic factor and targeting it is important in reducing angiogenesis. Bev...

Authors: Weina Li, Yonggang Ran, Meng Li, Kuo Zhang, Xin Qin, Xiaochang Xue, Cun Zhang, Qiang Hao, Wei Zhang and Yingqi Zhang

High level transient production of recombinant antibodies and antibody fusion proteins in HEK293 cells

The demand of monospecific high affinity binding reagents, particularly monoclonal antibodies, has been steadily increasing over the last years. Enhanced throughput of antibody generation has been addressed by...

Authors: Volker Jäger, Konrad Büssow, Andreas Wagner, Susanne Weber, Michael Hust, André Frenzel and Thomas Schirrmann

Self-assembled peptide amphiphiles function as multivalent binder with increased hemagglutinin affinity

A promising way in diagnostic and therapeutic applications is the development of peptide amphiphiles (PAs). Peptides with a palmitic acid alkylchain were designed and characterized to study the effect of the s...

Authors: Christine Hüttl, Cornelia Hettrich, Reinhard Miller, Bernd-Reiner Paulke, Petra Henklein, Harshadrai Rawel and Frank F Bier

Towards a novel influenza vaccine: engineering of hemagglutinin on a platform of adenovirus dodecahedron

The production process for the current influenza vaccine takes about 6 months and its antigenic composition must be modified annually. In the attempt towards developing influenza vaccine production that would ...

Authors: Antonina Naskalska, Ewa Szolajska, Igor Andreev, Malgorzata Podsiadla and Jadwiga Chroboczek

Modular glycosphere assays for high-throughput functional characterization of influenza viruses

The ongoing global efforts to control influenza epidemics and pandemics require high-throughput technologies to detect, quantify, and functionally characterize viral isolates. The 2009 influenza pandemic as we...

Authors: Sven N Hobbie, Karthik Viswanathan, Ido Bachelet, Udayanath Aich, Zachary Shriver, Vidya Subramanian, Rahul Raman and Ram Sasisekharan

Development of a recombinant antibody to target peptides and proteins to sialoadhesin-expressing macrophages

Sialoadhesin (Sn)-expressing monocytes/macrophages have been associated with several diseases like inflammatory and autoimmune disorders as well as viral infections, and they also appear to play a role in the ...

Authors: Karen Ooms, Hanne Van Gorp, Tim Van Gaever, Hans J Nauwynck and Peter L Delputte

Generation and functional characterization of the anti-transferrin receptor single-chain antibody-GAL4 (TfRscFv-GAL4) fusion protein

The development of vectors for cell-specific gene delivery is a major goal of gene therapeutic strategies. Transferrin receptor (TfR) is an endocytic receptor and identified as tumor relative specific due to i...

Authors: Qing Ye, Heyu Hu, Zhihua Wang, Tong Lu, Zhiquan Hu, Xing Zeng, Shu Zhang, Jing Liu, Ping Lei, Cong-Yi Wang, Zhangqun Ye and Guanxin Shen

Chemo-enzymatic synthesis and in vitro cytokine profiling of tailor-made oligofructosides

It is well known that carbohydrates play fundamental roles in cell signaling and infection processes as well as tumor formation and progression. However, the interaction pathways and cellular receptors targete...

Authors: Arne Homann, Malte Timm and Jürgen Seibel

Using protein microarray technology to screen anti-ERCC1 monoclonal antibodies for specificity and applications in pathology

An antibody with cross-reactivity can create unexpected side effects or false diagnostic reports if used for clinical purposes. ERCC1 is being explored as a predictive diagnostic biomarker for cisplatin-based ...

Authors: Donghui Ma, Dror Baruch, Youmin Shu, Kehu Yuan, Zairen Sun, Kaiyan Ma, Toan Hoang, Wei Fu, Li Min, Zhu-Sheng Lan, Fangxun Wang, Lori Mull and Wei-Wu He

A novel strategy for efficient production of anti-V3 human scFvs against HIV-1 clade C

Production of human monoclonal antibodies that exhibit broadly neutralizing activity is needed for preventing HIV-1 infection, however only a few such antibodies have been generated till date. Isolation of ant...

Authors: Rajesh Kumar, Raiees Andrabi, Ashutosh Tiwari, Somi Sankaran Prakash, Naveet Wig, Durgashree Dutta, Anurag Sankhyan, Lubina Khan, Subrata Sinha and Kalpana Luthra

Antigen vehiculization particles based on the Z protein of Junin virus

Arenavirus matrix protein Z plays an important role in virus budding and is able to generate enveloped virus-like-particles (VLPs) in absence of any other viral proteins. In these VLPs, Z protein is associated...

Authors: Cristina S Borio, Marcos F Bilen, Marcelo H Argüelles, Sandra E Goñi, Javier A Iserte, Graciela Glikmann and Mario E Lozano

Pandemic influenza vaccine: characterization of A/California/07/2009 (H1N1) recombinant hemagglutinin protein and insights into H1N1 antigen stability

The recent H1N1 influenza pandemic illustrated the shortcomings of the vaccine manufacturing process. The A/California/07/2009 H1N1 pandemic influenza vaccine or A(H1N1)pdm09 was available late and in short su...

Authors: Elena Feshchenko, David G Rhodes, Rachael Felberbaum, Clifton McPherson, Joseph A Rininger, Penny Post and Manon MJ Cox