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Effects of early geometric confinement on the transcriptomic profile of human cerebral organoids

Abstract

Background

Human cerebral organoids (hCO) are attractive systems due to their ability to model important brain regions and transcriptomics of early in vivo brain development. To date, they have been used to understand the effects of genetics and soluble factors on neurodevelopment. Interestingly, one of the main advantages of hCOs are that they provide three dimensionality that better mimics the in vivo environment; yet, despite this central feature it remains unclear how spatial and mechanical properties regulate hCO and neurodevelopment. While biophysical factors such as shape and mechanical forces are known to play crucial roles in stem cell differentiation, embryogenesis and neurodevelopment, much of this work investigated two dimensional systems or relied on correlative observations of native developing tissues in three dimensions. Using hCOs to establish links between spatial factors and neurodevelopment will require the use of new approaches and could reveal fundamental principles of brain organogenesis as well as improve hCOs as an experimental model.

Results

Here, we investigated the effects of early geometric confinements on transcriptomic changes during hCO differentiation. Using a custom and tunable agarose microwell platform we generated embryoid bodies (EB) of diverse shapes mimicking several structures from embryogenesis and neurodevelopment and then further differentiated those EBs to whole brain hCOs. Our results showed that the microwells did not have negative gross impacts on the ability of the hCOs to differentiate towards neural fates, and there were clear shape dependent effects on neural lineage specification. In particular we observed that non-spherical shapes showed signs of altered neurodevelopmental kinetics and favored the development of medial ganglionic eminence-associated brain regions and cell types over cortical regions. Transcriptomic analysis suggests these mechanotransducive effects may be mediated by integrin and Wnt signaling.

Conclusions

The findings presented here suggest a role for spatial factors in brain region specification during hCO development. Understanding these spatial patterning factors will not only improve understanding of in vivo development and differentiation, but also provide important handles with which to advance and improve control over human model systems for in vitro applications.

Background

Much of our understanding of brain development is and continues to be derived from foundational studies in animal models [1]. While these studies shed light into the general features of mammalian brain development, animal models do not capture the full complexity nor genetics of human neurodevelopment [2]. Recent progress with human cerebral organoid (hCO) systems show that these in vitro systems recapitulate many cell types and key features of the early in vivo brain making them attractive models to study human neurodevelopment [3,4,5,6,7,8,9,10,11]. As a model, they are continually being improved to better mimic in vivo architectures and brain regions and to enhance reproducibility, amongst other features. The development of any new understanding of factors regulating their development will therefore be important in advancing these models. Human fetal development is controlled by cascades of differential gene expression patterns regulated by not only morphogens, but also spatial and mechanotransducive factors. In fact, early neurodevelopment is one of the major processes where distinct tissue shapes like the neural tube emerge. Yet, while there is a relatively mature understanding of soluble factors and their effects on cerebral organoid development, the role and impacts of mechanobiological factors is unclear.

A strong body of literature has focused on three general approaches to understand mechanobiological contributions to neural development. Advanced light and force microscopy techniques have been used to observe and measure spatial and mechanical changes associated with the formation of the primitive streak and embryonic body axes and with neural tube closure in a variety of model organisms. This work revealed inhomogeneous strains and tensions arising throughout these processes [12, 13] Gross mechanical perturbations to embryos were also applied to live embryos and shown to alter gene expression patterns directly [14, 15]. This work has strongly suggested the role of shape and force in potentially regulating cell proliferation and differentiation and tissue patterning. Microcontact printing of extracellular matrix proteins and synthesis of hydrogels with defined rheological properties have therefore been leveraged to directly test the functional outcome of regulating cell and tissue shape and stiffness on proliferation and differentiation, largely in two-dimensional contexts. Interesting relationships have been identified in which softer environments and concave regions of lower cell–cell tensional forces within multicellular structures seem to support differentiation towards lineages of softer tissues like neurons [15,16,17,18,19,20]. Finally, genetic and pharmacological perturbations have implicated a variety of signaling pathways and molecular components transducing mechanical perturbations in neural systems, including integrins and GPCRs, focal adhesion proteins such as talin and vinculin, the RhoA/ROCK signaling axis, and Wnt/β-catenin and YAP/TAZ pathways. This work sets a strong foundation and identifies a key knowledge gap to fill. In particular, within a three-dimensional human context, how do shape perturbations affect organoid and neurodevelopment? While most work with hCOs to date has focused on the effects of soluble factors, the three-dimensional nature of hCOs provides a unique opportunity to explore the role of spatial and mechanical cues. Establishing links between mechanical cues and development will be an important step forward with regard to in vitro recapitulation of in vivo events, understanding how shape affects developmental changes, and improving organoid models [21].

Given the significant influence of spatial cues in directing human fetal development and hPSC differentiation, and prior work connecting embryoid body size with downstream spheroid and organoid development [22], we hypothesized that shape may also influence hCO development and brain region specification and that these changes would be reflected in differential gene expression patterns. To test this, we engineered agarose microwells with distinct shapes but equivalent volumes and seeded embryoid bodies (EB) within them. The EBs conformed to these shapes in the first few days of culture, and we proceeded to expose them to a whole brain hCO protocol that is highly permissive of natural patterning due to minimal addition of exogenous growth factors [23]. We analyzed the transcriptomic changes in hCOs at different differentiation stages in both conventional 96-well plates and the agarose microwells by RNAseq to investigate the potential impacts of microwell culture and initial EB shape on neuronal differentiation or regional specifications. Intriguingly, shapes that conferred additional mechanical strain elevated general neuronal differentiation as well as forebrain and midbrain gene expression patterns. This effect was not immediately apparent in the first week but became clear as the hCOs matured for 3 weeks in culture. Transcriptomic analysis indicates this effect was mediated through integrin and Wnt signaling. This work demonstrates that shape, while holding total cell number constant, regulates neural microtissue differentiation and could be an important regulatory handle for hCO engineering and downstream mechanistic mechanobiology studies on human neurodevelopment.

Results

Development and optimization of shape controlled hCOs in agarose microwells

The first step in the majority of current hCO protocols is the generation of embryoid bodies (EBs) and neuroectoderm from human pluripotent stem cells (hPSCs) [3, 24, 25]. Previous studies show that EBs are capable of developmental specification similar to that of the pre-gastrulation embryo and that the microenvironment and rigorous control of EB size strongly influence cell lineage-commitment [26,27,28,29,30,31,32]. In most hCO protocols, EB generation is carried out in low-attachment U- or V-bottom 96-well plates [23, 33] to form spherical aggregates of different sizes depending on the number of seeded cells.

While tissue geometry has previously been proposed as a potential factor regulating lineage commitment [16, 17, 31, 34], focus has been primarily on controlling EB size rather than shape especially in undirected differentiation protocols [27,28,29,30,31,32]. However, distinct shape changes occur during natural developmental processes. The primitive streak arising a couple weeks into development exhibits indentations and invaginations with thinning and thickening of different tissue layers, while the developing embryo rolls and curls during neural tube formation and closure. Here, we adapted a microengineering approach to fabricate distinctly shaped agarose microwells that mimic these changes. For example, invaginations and thinning/thickening were captured by peanut and butterfly shapes and curling was captured by “C”s [35] (Fig. 1a–d) (Additional file 1: Fig. S1A, B). PDMS devices did not perform well with cells and hCOs attaching to the device surfaces (Additional file 1: Fig. S1C). We aimed to capture the first 5 days of EB generation after cell seeding in microwells, during which media conditions allow all germ layers to form spontaneously [6, 31]. Several design parameters such as volume and shape geometry were optimized for formation of a single EB in each microwell similar to those that were generated in U-bottom 96-well plates (Fig. 1b–d).

Fig. 1
figure1

Engineering and optimization of early hCO shape-confinement in agarose microwells. a Schematic description of the agarose microwell fabrication (see also Methods for details). b Volume change of EBs seeded in a commercial 96-well plate. n = 20 EBs per time point. Error bars = 95% confidence intervals. c Size comparison of day 10 hCOs. n = 50 hCOs per shape. Error bars = 95% confidence intervals. d Development of EBs in distinctly shaped agarose microwells and 96-wells. e Images showing the shape retention of EBs. f Later developmental points of hCOs that were derived from EBs in microwells and 96-well plates. g Schematic of the experimental timeline

To minimize any size-related effects and ensure the resulting EBs in microwells were comparable in size to the EBs generated in 96-wells, we first calculated the volume changes over 5 days for EBs generated in 96-well plates (Fig. 1b). Then we adjusted the microwell design parameters and initial cell seeding density to achieve uniform EB sizes across all conditions (see also methods for details) (Fig. 1c). Next, we tested the efficiency of forming EBs in the different geometries. In addition to capturing increasing numbers of deformation points with peanuts and butterfly shapes and curling with C shapes, we also created shapes with increasing numbers of peripheral protrusions or corners such as the triangle, “A”, and star to assess the ability of EBs to conform to and fill in these more angular shapes. While EBs successfully formed in almost all tested shapes after 24-h, upon extended culture (3–4 days) we observed an effect where the EBs transiently shrunk in size, likely due to cell–cell adhesion, surface tension, and packing. The result was that in wells with peripheral protrusions or angular corners, the center of the shape provided more than enough room that the EB could exist as a fully round cell cluster without filling or touching the peripheral protrusions and hence did not ‘feel’ the well geometry (A, star and triangle). We therefore did not further pursue these geometries. In contrast, EBs in butterfly, peanut, C, and round microwells adopted the general shape of the microwell for 4 days and even 24-h after removal from the wells (Fig. 1d–e).

After 5 days, EBs were transferred from the microwell devices to low-attachment 24-well plates containing media driving neuroectoderm specification and hCO differentiation [23] (Fig. 1f-g). Comparisons of major/minor axis ratios showed no significant differences in shape by day 10 of differentiation and all hCOs had adopted a similar spherical shape (Fig. 1f) (Additional file 1: Fig. S1D). In addition, all tissues showed smooth edges with bright optically translucent peripheries consistent with healthy neuroectoderm formation. There was also evidence of optically clear neuroepithelial bud outgrowth after Matrigel embedding (Fig. 1f). These results indicated that hCOs initially seeded in shaped microwells (referred to as ‘microwell hCOs’ from here on) showed similar gross morphologies to hCOs developed in 96-well plates (referred as ‘96-well hCOs’ from here on), and they successfully met these developmental milestones of the whole brain hCO protocol followed in this study [23].

Microwell and 96-well hCOs show similar general developmental capacities

Gross morphological and size characteristics indicated that microwell culture were not likely having adverse effects on the ability of hCOs to form and differentiate towards neural lineages. To further confirm this, we characterized the global transcriptomic changes associated with time in culture in all samples using RNAseq analysis (Fig. 2). Principal component analysis (PCA) of samples from three time points (day 10, 20 and 40) identified notable transcriptional remodeling associated with differentiation with the greatest changes observed from day 10 to 40, and day 20 appearing as an expected transitional state (Fig. 2a, b) (Additional file1: Fig. S2A-B). Thus, for the remainder of our analysis we focused on the developmental changes between day 10 and day 40.

Fig. 2
figure2

Microwell and 96-well hCOs show similar general developmental capacities. ad n = 3 biological replicates for each condition with 4–10 hCOs comprising each replicate. a PCA of gene expression during hCO differentiation at days 10, 20 and 40. b The number of differentially expressed genes during hCO differentiation with false discovery rate (FDR) p-value < 0.05 and log2 (fold change) (FC) ≥|1.5|. c The top 35 differentially expressed genes between days 10 and 40 with FDR p-value < 0.05, log2(FC) ≥|1.5| and maximum mean reads per kilobase of transcript per million mapped reads (max mean RPKM) ≥ 10. d Top biological functions between days 40 and 10 with z-score ≥|2| and FDR p-value < 0.00001 identified through IPA analysis. Differentially expressed genes with FDR p-value < 0.05, log2 (FC) ≥|1.5| and max mean RPKM ≥ 10 were used in this analysis. 809 (96-well), 877 (Round), 621 (Butterfly), 807 (Peanut) and 788 (c) genes met this criteria

We focused on the top differentially expressed genes in 96-well hCOs and determined the changes for the same list of genes in microwell hCOs (Fig. 2c). This analysis revealed that in all samples as time in culture increased (day 40 versus 10) pluripotency associated genes (e.g., HES3, POU5F1, ZSCAN10, TDGF1, DPPA3, L1TD1, LIN28A, USP44, DNMT3B, HESX1) were downregulated, and neurodevelopment associated genes (e.g., TTR, TRIM67, TBR1, NSG2, SCG2, ISLR2, HTR2C, AQP1, STMN2, ELAVL3) were upregulated (Fig. 2c).

This developmental trend was also supported by Ingenuity Pathway Analysis (IPA) where all microwells and 96-well hCOs largely shared the same top IPA functions. While the primary functions predicted to be inhibited (z-score ≤ -2) were related to cell division (e.g., entry into interphase, alignment of chromosomes, synthesis of DNA), the functions predicted to be activated (z-score ≥ 2) were associated with neurodevelopment and cellular senescence (e.g., quantity of neurons, differentiation of nervous system, senescence) (Fig. 2d) (Additional file 1: Fig. S2C). Collectively, this analysis confirms that in addition to gross morphological similarities, transcriptional dynamics and overall differentiation programs during 96-well hCO development were conserved in microwell hCOs.

Early microwell culture affects subsequent hCO neurodevelopment

While the microwell conditions were similarly supportive of overall hCO development as the 96-well conditions were, there were transcriptomic differences apparent even in the initial PCA analysis (Fig. 2a). To identify potential changes associated with the microwell cultures in general, the transcriptomes of microwell hCOs were compared to 96-well hCOs at each time point. Resulting PCA analysis revealed clear transcriptomic differences between the microwell and 96-well environments (Fig. 3a). To capture the overall differences, we analyzed the differentially expressed genes between all microwell (regardless of the specific geometry) and 96-well hCOs. Notably, although the differences were apparent at all time points, they were particularly clear at day 40 (Fig. 3b).

Fig. 3
figure3

Early microwell culture affects subsequent hCO neurodevelopment. a PCA of gene expression at individual time points. n = 3 biological replicates for each condition with 4–8 hCOs in each replicate. b, c n = 12 biological replicates for microwell device (all shapes) with 4–8 hCOs in each replicate, n = 3 for 96-well plate with 4–8 hCOs in each. b The number of differentially expressed (up arrow) upregulated and (down arrow) downregulated genes between microwell device and 96-well plate with FDR p-value < 0.05 and log2(FC) ≥|1.5|. c Top biological functions between microwell device and 96-well plate with z-score ≥|2| and FDR p-value < 0.001 identified through IPA analysis. Differentially expressed genes with FDR p-value < 0.05 and log2 (FC) ≥|2| associated with each function are highlighted. Differentially expressed genes with FDR p-value < 0.05, log2 (FC) ≥|1.5| and max mean RPKM ≥ 5 were used in this analysis. 51 (day 10), 56 (day 20) and 166 (day 40) genes met this criteria

Based on the differentially expressed genes, IPA results predicted an activation (z-score ≥ 2) of functions related to nervous system development (e.g., quantity of nervous tissue, morphogenesis of neurons, migration of neurons) (Figs. 3c) (Additional file 1: Fig. S3) in microwell hCOs at all three time points. Common inhibited (z-score ≤ -2) functions were associated to organismal death (Fig. 3c) (Additional file 1: Fig. S3). Functions related to sensory system development were also inhibited at days 20 and 40. Interestingly, while some functions associated with excitatory-inhibitory balance such as seizures were inhibited, others such as coordination were activated in day 40 microwell hCOs.

Notable transcriptomic differences associated with these biological functions were; the stark downregulation of mesoderm (HAND1, > fivefold) [36] and pluripotency (UTF1, > twofold) [37] markers and the upregulation of dorsal medial ganglionic eminence (MGE) (SST, > 8 folds) [7, 9, 10] and intermediate progenitor markers (NEUROD1, NEUROG1, NHLH1, TFAP2B, all > 5 folds) [38, 39] at day 10, downregulation of retina associated genes (VSX2, SIX6, both > fourfold) [5] and upregulation of cortical (NEUROD2, NEUROD6, both > fourfold), forebrain (FOXG1, > threefold) and ganglionic eminence (GE) (DLX1, DLX2, DLX5, DLX6, SLC32A1, all > threefold) [7, 9, 10] markers at day 40 (Fig. 3c). These intriguing differential gene expression patterns and IPA results indicate that the initial EB growth environment and material selection (microwell versus 96-well) may instruct a lineage bias in subsequent neural tissue development.

Comparison of individual microwell geometries to 96-well hCOs reveal shape specific differences in gene expression

We then investigated how different microwell geometries contributed to the global transcriptomic differences observed in microwell and 96-well hCOs (Fig. 4) (Additional file 1: Fig. S4). Differential gene expression analyses between individual shapes and 96-well hCOs showed a varying trend of up and downregulated genes across the different geometries (Figs. 4a) (Additional file 1: Fig. S4A–C). This was particularly apparently at day 40 where round microwells showed an overall trend of downregulation, C microwells showed similar numbers of up and downregulated genes, and butterfly and peanut microwells were associated with upregulation of transcripts (Figs. 4a) (Additional file 1: Fig. S4C).

Fig. 4
figure4

Comparison of individual microwell shapes to 96-well derived hCOs reveal shape specific differences in gene expression. Ac n = 3 biological replicates for each condition with 4–10 hCOs in each replicate. a Volcano plots of differentially expressed genes between individual microwell shapes and 96-wells at day 40. Red highlighted genes: FDR p-value < 0.05, log2 (FC) ≥|2| and max mean RPKM ≥ 5. b Differentially expressed marker genes between individual microwell shapes and 96-wells with FDR p-value < 0.05. c Top biological functions between individual microwell shapes and 96-wells at day 40 with z-score ≥|2| and FDR p-value < 0.001 identified through IPA analysis. Differentially expressed genes with FDR p-value < 0.05, log2 (FC) ≥|1.5| and max mean RPKM ≥ 5 were used in this analysis. 174 (Round), 223 (Butterfly), 222 (Peanut) and 184 (c) genes met this criteria

To gain a deeper understand of these differences, we constructed a panel of established cell type and brain region marker genes [4, 6, 7, 9, 10, 33, 39, 40] (Fig. 4b). This analysis revealed that early EB growth in microwells instructed an early differentiation bias where genes associated with pluripotency and mesoderm were significantly downregulated in all microwell shapes at day 10. This was also accompanied by an upregulation in ectoderm marker SOX1. All microwell shapes showed downregulation of neural crest markers and upregulation of neural progenitor markers at day 20. Interestingly markers associated with intermediate progenitors and sensory neurons were only significantly upregulated in non-spherical shapes (butterfly, peanut and C). In addition, these non-spherical geometries also showed a drastic upregulation of MGE markers SST and DLX1 at day 10 followed by a robust upregulation of all MGE and lateral ganglionic eminence (LGE) associated genes at day 40. While some cortical markers (NEUROD2, NEUROD6) showed significant upregulation at day 40 in all shapes, a notable trend for cortical development was not observed across different shapes and time points. Similarly, floor plate, midbrain and hindbrain associated genes also showed varying results, whereas forebrain marker FOXG1 demonstrated significant upregulation in all microwell shapes at day 20 and 40. Furthermore, epithelial and mesenchymal fates and retinal development genes showed a notable downregulation in microwell hCOs (day 10 & 40 for epithelial, 20 and 40 for retina, all time points for mesenchymal markers).

These differences between microwells and 96-well and across different microwell geometries were also supported by IPA analyses (Fig. 4c) (Additional file 1: Fig. S4D-E). In agreement with the differential expression analysis, while round microwells showed an overall tendency for inhibition (z-score ≤ -2), the biological functions in butterfly, peanut and C were mostly associated with activation (z-score ≥ 2) at day 40 (Fig. 4c). These results demonstrate that while there are common features affected in all geometries indicating they could be the result of microwell growth environment, there are also differences specific to distinct geometries implying shape-associated transcriptomic differences in hCOs. Importantly, media, nutrient, and oxygen availability are likely different in the 96-well plate wells vs microwells due to more restricted access to hCOs in microwells; however, this availability was unlikely to be different between microwell geometries as the exposed surface areas and depths of the wells during the first four days of culture were identical for all geometries. The round microwells therefore served as the best control to identify geometry-specific effects on organoid development.

Microwell geometry modulates gene expression programs related to neural cell type and brain region specification

Having assessed that hCOs in all conditions were neuralizing appropriately and having characterized any basal effects of the agarose system compared to typical 96-well plates, we shifted our focus to the transcriptomic changes across different microwell geometries to investigate whether shape was informative of brain region specification (Fig. 5) (Additional file 1: Fig. S5). To isolate the shape specific effects from differences arising from microwell devices in general, round agarose microwells were used as the control group in this analysis and not 96-well plates.

Fig. 5
figure5

Microwell geometry modulates gene expression programs related to neural cell type and brain region specification. Ad n = 3 biological replicates for each condition with 4–10 hCOs in each replicate. a Venn diagrams showing the differentially expressed genes with FDR p-value < 0.05 and log2 (FC) ≥|1.5|. Boxed genes are the unique and common transcripts in each comparison with FDR p-value < 0.05, log2 (FC) ≥|2| and max mean RPKM ≥ 5. b The number of differentially expressed genes across all microwell geometries with FDR p-value < 0.05 and log2(FC) ≥|1.5|. c Differentially expressed marker genes between individual microwell geometries and round microwells with FDR p-value < 0.05. d Top biological functions between individual microwell geometries and round microwells with z-score ≥|2| and FDR p-value < 0.01 identified through IPA analysis. Differentially expressed genes with FDR p-value < 0.05, log2 (FC) ≥|1.5| and max mean RPKM ≥ 5 were used in this analysis. 149 (Butterfly), 28 (Peanut) and 35 (c) genes met this criteria in day 10 hCOs. 286 (Butterfly), 266 (Peanut) and 88 (c) genes met this criteria in day 20 hCOs. 106 (Butterfly), 69 (Peanut) and 10 (c) genes met these criteria in day 40 hCOs

Differential expression analysis revealed that while C microwells had the lowest number of differentially expressed genes at all time points, butterfly microwells showed the highest (Fig. 5a, b). Pairwise comparisons between all shapes demonstrated that peanut and C microwells did not show considerable transcriptomic differences from each other (Fig. 5b). While earlier differences observed between butterfly and peanut microwells were diminished at later points of differentiation, all shapes showed marked deviation from the round microwells at all time points (Fig. 5b), despite having the same exposed surface area and well depths.

The marker gene panel revealed several interesting differences between non-spherical shapes and round microwells. First, we noticed a robust upregulation at day 10 and a downregulation at day 20 for markers associated with neural differentiation (neural precursors, intermediate progenitors and neurons) which applied to all shapes (Fig. 5c). To understand the basis of this pattern, we re-examined our previous comparison between distinct microwell shapes and 96-well hCOs (Fig. 4b). This analysis revealed that while butterfly, peanut and C microwells showed upregulation in these markers at day 10, the same change was not observed in round microwells until day 20 (Fig. 4b) which may imply that the neural differentiation timeline is lengthened in round microwells (or accelerated in non-spherical shapes). A similar effect was also observed for LGE and axon guidance markers. This could reflect that geometry can help cell fate specification and developmental programs to be turned on, and that typical spherical cultures may therefore be less ‘complete’ in providing developmental signals.

There were also cell type and brain region specific differences between shapes (Fig. 5c). Notably, we observed an overall downregulation of cerebral cortex, forebrain, midbrain, choroid plexus and mesenchymal markers in non-spherical shapes (Fig. 5c). In contrast, MGE, floor plate, hindbrain, epithelial and extracellular matrix markers were upregulated in these shapes (Fig. 5c). These results may indicate that while round microwells promote cortical fates, other shapes favor MGE-associated brain regions and cell types. Overall, the most drastic changes were observed in butterfly microwells, followed by peanut and C geometries, perhaps reflecting the relative extent of deformation points of each shape with butterfly microwells having the most (four) indentations. Supporting these observations, IPA results also highlighted butterfly microwells as the shape that was associated with the most significant alterations in biological functions (Figs. 5d) (Additional file 1: S5). This analysis also revealed that there was an overall inhibition (z-score ≤ -2) of cell death related functions (e.g., organismal death, apoptosis) and activation (z-score ≥ 2) of cell viability linked functions (e.g., quantity of cells, cell viability, cell viability of neurons) in non-spherical shapes (Fig. 5d).

Mechanotransduction-associated genes perturbed by hCO geometry

In comparing the different geometries to round microwells, IPA highlighted functions related to cellular mechanics such as cell adhesion, migration of cells, cell movement, invasion of cells and microtubule dynamics (Fig. 5d) (Additional file 1: S5). This suggested that mechanotransducive mechanisms may indeed be driving the effects of shape on hCO gene expression. We therefore asked what pathways or molecular components might be mediating shape-driven changes in hCO gene expression. Prior work investigating mechanoresponsive mechanisms in stem cell systems have implicated a variety of pathways and signaling components. From this work and the broader mechanobiology literature, we curated a list of 98 mechanotransduction-associated genes including Wnt and Notch-Delta pathway genes, integrins, twist, brachury, SOX3, β-catenin, VCL, ZYX, TLN, PTK2, PIK3ca, YAP/TAZ, the Rho GTPases, ROCK, and myosins, among others (Fig. 6) (Additional file 4). To investigate the immediate downstream effects of the geometrical perturbation, we focused on the expression of these genes on day 10 as it was the first collection point after hCOs were removed from the microwells (Fig. 6).

Fig. 6
figure6

Mechanotransduction-associated genes perturbed by hCO geometry. Differentially expressed mechanotransduction-associated genes with FDR p-value < 0.01, log2(FC) ≥|1.5| and max mean RPKM ≥ 0.5 in day 10 hCOs

By comparing all microwell shapes as well as the 96-well condition to the round microwells, it became evident that shape perturbations enriched for multiple Integrin, Delta-like, and Wnt pathway genes (Fig. 6). The number of differentially expressed mechanotransducive genes from our curated list of 98 genes was also greater in butterfly microwells (13) than C and peanut microwells (8 each), consistent with butterfly microwells having more mechanical perturbations/indentations. In contrast, the difference in 96-well versus agarose microwell culture systems enriched for only one gene out of the 98 we curated.

Discussion

During the past decade, various in vitro strategies have been developed to study human brain development [41]. Among these, recently developed hCO systems are particularly advantageous due to their ability to capture important brain regions and cell types [2, 3]. While the primary focus in the field has been on identifying appropriate soluble factor cocktails to drive hCO formation, the present work provides a pilot analysis dedicated to the evaluation of geometric confinement effects on hCO development. This work also extends prior work investigating mechanobiological and shape-based influences on stem cell differentiation in two-dimensional systems to human three-dimensional organoids.

Our analysis comparing the microwell growth environment to 96-wells (Figs. 3, 4) showed apparent differences between transcriptional profiles including the stark downregulation of pluripotency markers, mesoderm, retina associated genes and mesenchymal fates in all microwell hCOs. Although, the microwell parameters were optimized with rigorous calculations and experimentation of several different conditions, these overall changes could be due simply to the confined geometric environment in the microwells compared to the unconfined U-bottom 96-well plates and the inherent material specific differences. In fact, material properties have previously been shown to affect cell fate decisions [42]. While agarose is a non-toxic material widely used in cell culture studies [43], the distinct surface or chemical properties between poly-styrene (96-wells) and agarose (microwells) may contribute to the transcriptomic differences observed in this study and make any comparisons between 96-well and microwell plates difficult. Therefore, in this study, the shape-related analysis was limited only to the hCOs grown in microwells, and the round microwells was used as the control group. In addition, unlike the U-bottom 96-well plates, the microwells had flat bottoms due to the fabrication method. While concave microwells have been used by others [28], their effects on differentiation in comparison to flat-bottomed wells, and in the context of shape, have not been investigated. Future studies could revise the fabrication methods used in this study to fabricate concave microwells to eliminate the potential transcriptomic differences that could result.

All of the non-spherical shapes investigated in this study showed similar patterns of differential gene expression compared to round microwells (Figs. 5, 6). However, these changes, including the mechanotransduction-associated genes, were more pronounced in butterfly shaped microwells, followed closely by the peanut microwells. C shape microwells showed the closest transcriptional profile to the round microwells. Interestingly, this pattern correlates with the number of deformation points applied to EBs in each geometry. Future work could fine tune these shapes to achieve uniformity across all other parameters and specifically control for the number of deformation points. In addition, simulations or molecular force sensors [44] could be used to determine shape-dependent stress localizations in different geometries. Comparing similar geometries with slightly different parameters such as size, curvature, and major over minor axis ratios could help elucidate the specific shape-related bases of these transcriptional differences.

Among the differences we observed across shapes, the stark upregulation of dorsal MGE marker SST at day 10 followed by other hallmark MGE markers such as NKX2-1, DLX1/2, GAD1 and SLC32A1 (also known as VGAT) were the most notable changes. Coupled with the downregulation of cortical markers such as TBR1, NEUROD2/6 and EMX1/2, this pattern in non-spherical shapes resembled the previously described MGE [7] and ventral forebrain hCOs [9, 10]. Interestingly, while the whole brain hCO differentiation protocol followed in this study [6, 23] enables the generation of multiple different brain regions, dorsal cortical regions are more frequently identified compared to ventral fates [6, 41, 45]. Thus, shape could provide a new means with which to tune the dorsal–ventral specification of hCOs.

Future work could investigate in greater depth the molecular mechanisms by which shape guides hCO development and, in particular, how geometrical deformations increase MGE and ventral fates. Here we observed that geometrical deformations led to upregulation of integrin, Wnt, and Delta-related genes. While Wnt is typically thought to promote dorsal fates, there are multiple Wnt signaling pathways, some of which are associated with ventral fates as well including Wnt 5a/7a/7b [46]. hCOs could provide a highly tractable system to functionally dissect the roles of the different Wnt family members on regional specification. Similarly, there are many integrins and pairings of integrin receptors. Here we saw upregulation of integrins α9 and β6 in geometrically perturbed microwell hCOs. These could serve as initial targets to investigate using specific agonists, antagonists or genetic approaches. Finally, Delta-like genes were also upregulated in hCOs grown in butterfly microwells suggesting multiple pathways may be involved in transducing geometrical perturbations. Further validation studies with immunohistochemistry will be needed to confirm these changes at the protein level. It is also not clear that transcriptomic changes in mechanotransductive components are necessary to transduce geometrical perturbations, and the possibility that these changes may be compensatory due to a reduction in the stimulation of these pathways should also be considered. Overall, these results present several avenues for future detailed mechanistic studies, and they could reveal key insights into mechanobiological regulation of neurodevelopmental while also enhancing the engineering of hCOs as an experimental model.

Conclusions

Here, we used agarose microwells to control EB shapes and generated whole brain hCOs from these EBs to investigate the effects of early spatial regulation on transcriptomic changes during brain region specification.

Our results showed that gross morphological and transcriptional changes related to hCO differentiation were similar between 96-well and microwell hCOs, indicating that the microwell growth environment did not significantly affect the overall neural differentiation or viability of the hCOs (Figs. 1, 2). However, investigating individual developmental time points and comparing across 96-well and microwell conditions more closely revealed notable differences in genes associated with pluripotency, mesoderm, MGE, retina and cortical development, and epithelial and mesenchymal fates (Fig. 3), implying that the microwell growth environment and material may bias lineage commitment in neurodevelopment and that round microwells were necessary as an appropriate control to specifically parse geometrical effects. Furthermore, we found that the direction and intensity of this transcriptional bias was associated with the starting EB shapes (Figs. 4, 5). The butterfly shape showed the most noticeable differences, followed closely by the peanut shape. Among other notable differences, round microwells exhibited a potential delay in the kinetics of neurodevelopment, and our results also demonstrated that non-spherical shapes may favor the development of MGE-associated brain regions and cell types over cortical regions (Fig. 5). Finally, we identified integrins, Wnt, and Delta-like pathway genes were differentially expressed specifically as a result of microwell shape and not due to any difference in tissue culture plastic versus agarose (Fig. 6). These indicate potential mechanistic pathways that could be transducing geometrical signals and warrant further pharmacological and genetic investigation.

The findings presented here suggest a role for mechanobiological factors in brain region specification and hCO development. Uncovering how fate decisions are tied to these early spatial changes could provide a better understanding of how embryonic morphogenetic processes are orchestrated. In addition, we believe that these results not only offer a new perspective to advance our biophysical and molecular understanding of human brain development, but also pave the way for new experimental directions to refine the engineering of in vitro model systems and organoids.

Methods

Agarose microwell device fabrication

Shapes were designed using Autocad (Autodesk) and photomasks were printed using Fineline Imaging printing services. The silicon wafer with these shapes was fabricated using photolithography [47]. Briefly, molds were fabricated by coating SU-8 2150 photoresist with a Laurell spin coater at 500 rpm for 7 s then at 1000 rpm for 35 s. Following photolithography, agarose microwells were fabricated by replica-molding (Fig. 1a) (Additional file 1: Fig. S1A–B) [35]. Briefly, using a 10 cm circular stencil, a 1 cm layer of 2% agarose in PBS was poured onto the silicon wafer. Following this, air bubbles were removed by degassing and agarose was cooled at room temperature for 30 min to allow for congealing. Then, the agarose disc was gently removed and placed into a sterile 6-well culture plate, with the imprinted patterns facing upward. A cut pipette tip was placed on the pattern, so that roughly 80 patterned microwells were enclosed in the tip. With the pipette tip in place, warm agarose was poured in the remainder of the well. After the second layer of agarose cooled and congealed, the pipette tip was removed, leaving a small enclosure with shaped microwells (Fig. 1a) (Additional file 1: Fig. S1B). Then, 2 mL of 1 × PBS was added to the agarose device and it was sterilized under UV light overnight. Base surface area and height of microwells were 0.117 mm2 and 0.6 mm, respectively, to achieve a 0.07 mm3 total volume in the microwells.

Cell culture and human cerebral organoid generation

Feeder-independent H9 human embryonic stem cells (hESCs) (WA09) were obtained from WiCell. Cells were maintained in tissue culture dishes (Fisher Scientific Corning Costar) coated with 0.5 mg/cm2 Vitronectin (VTN-N) (Thermo Fisher Scientific) in E8 medium (Thermo Fisher Scientific) and passaged using standard protocols. 96-well and microwell human cerebral organoids (hCOs) were generated using the same pool of hESCs and differentiated using the same protocol as described [6, 23]. Number of cells seeded per well was experimentally optimized for successful generation of EBs in microwells. For each microwell device, 60 μL of hESC suspension at 32,000 cells/well was pipetted into the microwell enclosure using a cut p200 tip. The device was gently placed into the incubator for 5 min to allow cells to settle into the microwells. After 5 min, 2.5 mL media was slowly added to the wells avoiding the disruption of the seeded cells in the microwells. Media was changed every 12 h for the first 3 days with fresh hESC medium with basic fibroblast growth factor (bFGF) (Invitrogen) and ROCK inhibitor (Y-27632, LC laboratories) for all hCOs (microwell and 96-well). All cells and hCOs were maintained in a humid incubator at 37 °C with 5% CO2. After 5 days all hCOs were transferred into low-attachment 24-wells plates (1 hCO/well) and the remainder of the previously described whole brain organoid protocol was followed [6, 23].

Total RNA extraction and sequencing

For RNA extraction the following samples were collected from three independent culture plates: 10 pooled hCOs for day 10 samples, 6 pooled hCOs for day 20 samples, and 4 pooled hCOs for day 40 samples. hCOs were washed 3 times in cold PBS. Total RNA was extracted as previously described [48]. Briefly, Matrigel was dissolved by incubating the hCOs in chilled Cell Recovery Solution (Corning, cat. no. 354253) for 1 h at 4 °C. The dissolved Matrigel was removed by rinsing 3 times in cold PBS. Total RNA was isolated using Direct-zol RNA MicroPrep Kit (Zymo Research) according to the manufacturer’s protocol. RNA samples were collected in 2 mL RNAse-free tubes and chilled on ice throughout the procedure. Bulk RNA was sequenced using DNB-seq PE100 platform (BGI).

RNAseq analysis

Raw FASTQ formatted sequence reads were imported into CLC Genomics Workbench (version 20.0.2, QIAGEN Digital Insights). Adaptor sequences and bases with low quality were trimmed and reads were mapped to the reference genome (GRCh38.102) using the RNAseq analysis tool with the default parameters recommended for RNAseq analysis. Principal component analysis and differential expression analysis were performed using ‘PCA for RNAseq’ and ‘Differential Expression for RNA-seq’ toolsets. ‘Wing’ like patterns observed in volcano plots reflect the mathematical relationship between fold change and p-values. This was exposed in our data set due to the number of replicates that were used, and the genes that appeared in these regions were not included in the analyses. Heatmaps were generated using Euclidean distance with complete linkage. The top 10,000 features with highest coefficients of variance were displayed. Significance was selected as log2(fold change) ≥|1.5| in expression between comparison groups with a threshold false discovery rate (FDR) adjusted p-value < 0.05. All sequencing data passed default quality filters for the CLC Genomic Workbench version 20.0.2 RNA-Seq pipeline analysis (sequencing statistics summary available upon request). FASTQ sequencing files and sample metadata are publicly available under the Gene Expression Omnibus (GEO) accession GSE167208.

Ingenuity Pathway Analysis (IPA, version 60467501, QIAGEN Digital Insights) was used to predict activated or inhibited biological functions. Differentially expressed genes with log2(fold change) ≥|1.5|, FDR p-value < 0.05, maximum mean reads per kilobase of transcript per million mapped reads (max mean RPKM) ≥ 10 (Fig. 2) and max mean RPKM ≥ 5 (Figs. 3, 4, 5) were used in IPA. Statistical z-score (≥ 2 for activation and ≤ -2 for inhibition) was used to identify the predicted activity status of significant (FDR p-value < 0.05) biological functions. Cancer related biological functions were excluded from the analyses.

Availability of data and materials

The datasets generated and/or analyzed during the current study are available from the corresponding author upon reasonable request. The bulk RNA seq data reported in this paper is publicly available under GEO accession GSE167208 (https://0-www-ncbi-nlm-nih-gov.brum.beds.ac.uk/geo/query/acc.cgi?acc=GSE167208).

Abbreviations

hCO:

Human cerebral organoid

EB:

Embryoid body

MGE:

Medial ganglionic eminence

SHH:

Sonic hedgehog

IPA:

Ingenuity Pathway Analysis

RNAseq:

RNA sequencing

PDMS:

Polydimethylsiloxane

FDR:

False discovery rate

RPKM:

Read per kilobase of transcript per million mapped reads

VTN-N:

Vitronectin

hESC:

Human embryonic stem cell

bFGF:

Basic fibroblast growth factor

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Acknowledgements

We thank Drs. Adriana San Miguel and Sahand Saberi-Bosari for their valuable training in microfabrication process and allowing us to use their equipment.

Funding

This work was supported by a Simons Foundation SFARI Explorers Grant (495112), the NCSU Faculty Research and Professional Development Program, an NCSU Research Innovation Seed Fund Grant, the NSF Emerging Frontiers in Research and Innovation program (NSF-1830910), an NIH Avenir Award (DP1-DA044359), Foundation for Angelman Syndrome Therapeutics Targeted Research to Advance a Cure Grant (FT2020-003), and a fellowship from the American Association of University Women (to DS). These funding bodies did not play a role in the design of the study and collection, analysis, and interpretation of data and in writing the manuscript.

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DS, AV and AJK conceived the study. DS and AV planned and performed the wet lab experiments with guidance from AJK. DS performed the RNAseq analysis. DS and AJK wrote the paper. All authors read and approved the final manuscript.

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Correspondence to Albert J. Keung.

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Supplementary Information

Additional file 1

. Supplementary figures S1-5. Extended data related to main figures 1-5, with legends.

Additional file 2

. PDF version of AutoCAD (DWG) file related to main figure 1. Schematic files for the designs of the microwells.

Additional file 3

. PDF version of AutoCAD (DXF) file related to main figure 1.Schematic files for the designs of the microwells.

Additional file 4

. Excel file related to main figure 6. Extended list of mechanotransduction-associated genes investigated in this study.

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Sen, D., Voulgaropoulos, A. & Keung, A.J. Effects of early geometric confinement on the transcriptomic profile of human cerebral organoids. BMC Biotechnol 21, 59 (2021). https://0-doi-org.brum.beds.ac.uk/10.1186/s12896-021-00718-2

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Keywords

  • Human cerebral organoids
  • Neurodevelopment
  • Agarose microwells
  • Microfabrication
  • RNAseq
  • Mechanobiology