Fig. 2
Cleavage efficiencies of GGN18- and GGN20-format sgRNAs targeting BmBLOS2. A BLOS2-T1 was targeted by sgRNAs in the GGN18 or GGN20-formats. A T7EN1 reaction was performed to evaluate cleavage efficiencies, and the products were separated by PAGE. The intact target is 600 bp in length, and the two bands derived from T7EN1 cutting are approximately 311 and 289 bp. The extra band that appears between 311 and 289 bp bands may be derived from SNPs in the amplified 600 bp region. The wildtype (WT) controls were amplified from Dazao genomic DNA by PCR. B Cleavage efficiencies were calculated by measuring band intensities. Bars represent mean values, and error bars represent SEM. P-values were determined using Student’s t-test. *P < 0.05. Please note that the uncropped original PAGE gel images are shown in Additional file 1: Figure S3