Fig. 5From: Active-site engineering of ω-transaminase from Ochrobactrum anthropi for preparation of L-2-aminobutyric acidSmall-scale preparation of L-ABA with OATAL57C/M419I and TD. The reaction mixture (50 mL) contained 0.5 mM PLP, TD (0.2 mg/mL), OATA (1 mg/mL), 300 mM L-threonine, 450 mM isopropylamine and 50 mM phosphate buffer (pH 7.5). The reaction was carried out at 37 °C for 30 minBack to article page