Fig. 4From: Active-site engineering of ω-transaminase from Ochrobactrum anthropi for preparation of L-2-aminobutyric acidEffect of mutations on enzyme stability. Purified enzymes were incubated in 50 mM phosphate buffer (pH 7.5) with 2.5 mg/mL OATA at 37 °C. The reaction mixture (100 μL) contained 0.5 mM PLP, OATA (0.25 mg/mL), 300 mM α-ketobutyric acid, 450 mM isopropylamine and 50 mM phosphate buffer (pH 7.5). The reaction was carried out at 37 °C for 30 minBack to article page