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Table 2 Comparison of slop, amplification efficiency, and R2 estimated through the linear regression of triple standard curves of Cel-miRNA-39-1 according to the annealing temperature

From: Screening of miRNAs in plasma as a diagnostic biomarker for cardiac disease based on optimization of extraction and qRT-PCR condition assay through amplification efficiency

Parameters

Annealing temp. (℃)

58

60

62

Slope

 Median

− 4.144

− 3.554

− 3.648

 Range

− 4.5042 to − 3.8800

− 3.5939 to − 3.3490

− 4.1735 to − 3.6066

 95% CI

− 4.953 to − 3.396

− 3.825 to − 3.173

− 4.594 to − 3.024

Efficiency (%)

 Median

74.40

91.15

62.01

 Range

66.7 to 81.0

89.8 to 98.9

73.6 to 89.3

 95% CI

56.29 to 91.81

81.08 to 105.5

62.01 to 105.3

R2

 Median

0.9970

0.9993

0.9962

 Range

0.9820 to 0.9993

0.9907 to 0.9999

0.9899 to 0.9973

 95% CI

0.9696 to 1.016

0.9838 to 1.009

0.9845 to 1.004