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Fig. 4 | BMC Biotechnology

Fig. 4

From: Screening of miRNAs in plasma as a diagnostic biomarker for cardiac disease based on optimization of extraction and qRT-PCR condition assay through amplification efficiency

Fig. 4

Efficiency of RNA extraction for endogenous miRNA-16 detection from plasma by qRT-PCR in different transferred upper phase volumes. Results represent the raw Cq values obtained for miRNA-16. RNA samples from plasma were obtained by extraction with Trizol LS in single chloroform extraction and without ethanol washing. In the figure, dots represent individual Cq values, the horizontal bar is the group mean. Statistical differences were determined using Kruskal–Wallis test followed by Dunn’s post hoc test for multiple comparisons (*P = 0.0216)

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BMC Biotechnology

ISSN: 1472-6750

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