Table 2 Median DNA yield (ng/μL), total DNA yield (ng), and quality (A260/280 and A260/230) of 11 extraction protocols tested on 6 individual adult, female Teladorsagia circumcincta specimens
Extraction Method | Median (Range) Invitrogen Qubit™ DNA Concentration (ng/μl) | Median (Range) Invitrogen Qubit™ DNA Total Yield (ng) | Median (Range) NanoDrop 2000™ DNA Concentration (ng/μl) | Median (Range) NanoDrop 2000™ DNA Total Yield (ng) | Median (Range) NanoDrop 2000™ DNA Quality (A260/280) | Median (Range) NanoDrop 2000™ DNA Quality (A260/230) |
|---|---|---|---|---|---|---|
AccM | 0.0897 (0.0228–0.178) | 4.485 (2.12–8.9) | 0.9 (0.6–1.4) | 45 (30–70) | 0.935 (−13.1–1.64) | 0.53 (0.18–0.73) |
AccW | 0.08125 (0.0129–0.127) | 4.0625 (0.645–6.35) | 1.3 (0.9–2.8) | 65 (45–140) | 1.73 (− 5.76–3.58) | 0.77 (0.62–1.52) |
CheX | 0.98 (0.429–1.64) | 49 (24.6–82) | 11.5 (9.3–16.5) | 575 (465–825) | 1.99 (1.63–2.37) | 0.535 (0.46–0.67) |
CTAB | Undetectable | Undetectable | 0.7 (−1.2–1.1) | 35 (− 60–55) | 0.675 (− 13.83–1.51) | 0.395 (−1.02–0.98) |
EznF | 0.4325 (0.142–0.672) | 21.625 (7.1–40) | 4.85 (3.2–11.5) | 242.5 (160–575) | 2.375 (1.99–2.83) | 1.305 (0.84–2.34) |
IsoG | 0.2035 (0.109–0.372) | 10.175 (5.45–18.15) | 1.9 (1.0–3.0) | 95 (50–150) | 1.87 (− 24.56–3.27) | 1.545 (0.52–3.51) |
Schi | 0.962 (0.58–1.51) | 48.1 (29–75.7) | 6.4 (4.1–8.4) | 320 (205–420) | 2.19 (1.68–2.77) | 0.99 (0.46–2.77) |
Schi-LE | 0.064 (undetectable – 0.203) | 3.08 (undetectable – 10.15) | 1.25 (0.5–2.3) | 62.5 (25–115) | 2.12 (− 6.04–5.39) | 0.245 (0.08–0.54) |
SDS | 0.16 (undetectable – 0.236) | 7.425 (undetectable – 11.8) | 4.1 (3.3–10.1) | 205 (165–505) | 3.925 (− 33.69–10.76) | 0.05 (0.03–0.11) |
WizM | 0.0079 (undetectable – 0.0079) | 0 (0–0.395) | 0.45 (0.2–0.9) | 22.5 (10–45) | 0.985 (− 3.65–3.78) | − 0.005 (− 1.4–0.42) |
WizP | Undetectable | Undetectable | 1.4 (0.9–1.8) | 70 (45–90) | 0.505 (− 12.31–17.77) | 0.465 (0.26–1.06) |