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Fig. 5 | BMC Biotechnology

Fig. 5

From: Comparative evaluation of different molecular methods for DNA extraction from individual Teladorsagia circumcincta nematodes

Fig. 5

Agarose gels displaying amplicons produced by conventional PCR using primer pair ITS-2/NC2. Genomic DNA samples from single Teladorsagia circumcincta nematode specimens. Ladder indicating amplicon size (bp) (lanes 1, 14, 27). a Schi (lanes 2–7), Schi-LE (lanes 8–13), SDS (lanes 15–20), CheX (lanes 21–26), no-DNA control (lane 28), T. circumcincta ITS-2 gBlocks™ Gene Fragment (Integrated DNA Technologies) control (lane 29). b WizP (lanes 2–7), EznF (lanes 8–13), AccM (lanes 15–20), IsoG (lanes 21–26), no-DNA control (lane 28), T. circumcincta ITS-2 gBlocks™ Gene Fragment control (lane 29). C: AccW (lanes 2–7), WizM (lanes 8–13), CTAB (lanes 15–20), no-DNA control (lane 21), T. circumcincta ITS-2 gBlocks™ Gene Fragment control (lane 22). The specificity of individual amplicons produced was verified by direct sequencing

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