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Fig. 4 | BMC Biotechnology

Fig. 4

From: Comparative evaluation of different molecular methods for DNA extraction from individual Teladorsagia circumcincta nematodes

Fig. 4

Box-and-whisker plots of DNA quality measured by ThermoScientific NanoDrop 2000™ absorbency measurement 260 nm / 230 nm ratios. 11 DNA extraction protocols were used, each with 6 individual adult, female T. circumcincta specimens. Boxes extend from the 25th to the 75th percentile of each group’s distribution of values; Whiskers above and below the box indicate the 10th and 90th percentiles. The red line indicates the desired absorbency ratio of 2.0. Median line __; Mean □; Outliers ♦; Dunn’s Multiple Comparison test significance between methods indicated by asterisk; *: 0.01 < p < 0.05; **: 0.001 < p < 0.01; ***: 0.0001 < p < 0.001. Extraction method abbreviations: AccM (AccuPrep® Genomic DNA Extraction - Mammalian Tissue), AccW (AccuPrep® Genomic DNA Extraction Kit – Whole Blood, Buffy Coat and Cultured Cells), CheX (Chelex®100), CTAB (cetyl trimethyl ammonium bromide), EznF (E.Z.N.A.® Forensic DNA), IsoG (Isolate II Genomic DNA Kit), Schi (Schistosoma sp. DNA Extraction Method), Schi-LE (Schi with larval exsheathment), SDS (sodium dodecyl sulphate), WizM (Wizard® Genomic DNA Purification Kit - Mouse Tail), WizP (Wizard® Genomic DNA Purification - Plant Tissue)

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