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Table 3 List of primers

From: Rational design and structure-based engineering of alkaline pectate lyase from Paenibacillus sp. 0602 to improve thermostability

Name

Sequence

Description

PelN_F

GGAATTCCATATGGCGGGCAATGCAG

Nde1, PelN 5′

PelN_R

CCCTCGAGATAGCTCGTCTTC

Xho1, PelN 3′

G90V_F

GTGTTGAAGCAGACTGTAGTCAGCAAAA

G90V

G90V_R

CGTAATTTTGCTGACTACAGTCTGCTTC

G90V

S92F_F

AAGCAGACTGGTGTCTTCAAAATTACGG

S92F

S92F_R

GTCCACCGTAATTTTGAAGACACCAGTC

S92F

E137Y_F

TTCGATGAGCTGTGGTACTGGGATGAGT

E137Y

E137Y_R

GGTGGACTCATCCCAGTACCACAGCTCA

E137Y

E137F_F

TTCGATGAGCTGTGGTTCTGGGATGAGT

E137F

E137F_R

GGTGGACTCATCCCAGAACCACAGCTCA

E137F

E137W_F

TTCGATGAGCTGTGGTGGTGGGATGAGT

E137W

E137W_R

GGTGGACTCATCCCACCACCACAGCTCA

E137W

D178F_F

TATGACGGACTTGTGTTCTCGAAAAAAG

D178F

D178F_R

GGTTCCTTTTTTCGAGAACACAAGTCCG

D178F

D178Y_F

TATGACGGACTTGTGTACTCGAAAAAAG

D178Y

D178Y_R

GGTTCCTTTTTTCGAGTACACAAGTCCG

D178Y

D178C_F

TATGACGGACTTGTGTGCTCGAAAAAAG

D178C

D178C_R

GGTTCCTTTTTTCGAGCACACAAGTCCG

D178C

D178V_F

TATGACGGACTTGTGGTATCGAAAAAAG

D178V

D178V_R

GGTTCCTTTTTTCGATACCACAAGTCCG

D178V

G241A_F

ATTATTGCCATCTCTGCGTCGCAGAAAA

G241A

G241A_R

ACCTTTTTTCTGCGACGCAGAGATGGCA

G241A

G241V_F

ATTATTGCCATCTCTGTATCGCAGAAAA

G241V

G241V_R

ACCTTTTTTCTGCGATACAGAGATGGCA

G241V

G246W_F

GGTTCGCAGAAAAAATGGCATCTTGTCG

G246W

G246W_R

CGCACCGACAAGATGCCATTTTTTCTGC

G246W

G325W_F

GGGATTACCAGCAACTGGGCTATTTCCA

G325W

G325W_R

TTCAGTGGAAATAGCCCAGTTGCTGGTA

G325W

  1. Restriction sites are underlined and mutated nucleotides are in bold