Table 2 Design matrix and response for the selected three variables in the Box–Behnken design for α-amylase production using groundnut, coconut, and sesame oil cake as substrates
Run | pH (A) | Incubation temperature (B) (°C) | Incubation period (h) | α-Amylase activity (U*/gds) | ||
|---|---|---|---|---|---|---|
GOC | COC | SOC | ||||
1 | 3 | 25.0 | 108 | 2327.00 | 1775.70 | 1423.70 |
2 | 6 | 25.0 | 108 | 4549.00 | 3112.50 | 2371.85 |
3 | 3 | 40.0 | 108 | 1586.60 | 1086.27 | 720.00 |
4 | 6 | 40.0 | 108 | 1568.00 | 1059.67 | 985.24 |
5 | 3 | 32.5 | 72 | 6031.00 | 3068.00 | 2660.74 |
6 | 6 | 32.5 | 72 | 8519.20 | 2327.40 | 1475.56 |
7 | 3 | 32.5 | 144 | 5290.30 | 3512.59 | 2934.81 |
8 | 6 | 32.5 | 144 | 6549.00 | 2075.50 | 1475.56 |
9 | 4.5 | 25.0 | 72 | 5305.00 | 2238.50 | 2194.07 |
10 | 4.5 | 40.0 | 72 | 2102.50 | 1122.86 | 850.00 |
11 | 4.5 | 25.0 | 144 | 3912.00 | 2148.00 | 2031.10 |
12 | 4.5 | 40.0 | 144 | 1898.00 | 1102.60 | 896.00 |
13 | 4.5 | 32.5 | 108 | 9868.12 | 4031.10 | 3068.15 |
14 | 4.5 | 32.5 | 108 | 9868.00 | 4031.10 | 3068.15 |
15 | 4.5 | 32.5 | 108 | 9864.50 | 4031.10 | 3068.15 |
16 | 4.5 | 32.5 | 108 | 9868.00 | 4031.10 | 3068.15 |
17 | 4.5 | 32.5 | 108 | 9868.00 | 4031.10 | 3068.15 |