Skip to main content
Fig. 3 | BMC Biotechnology

Fig. 3

From: Optimizations for identifying reference genes in bone and cartilage bioengineering

Fig. 3

V0.15- vs. Vmin-based normalization scheme for gene expression assay on muscle tissue undergoing osteogenic differentiation. a Pairwise variation (Vn/n + 1) analysis between NFn and NFn + 1 to determine the optimal number of reference genes required for accurate normalization, V0.15 = 0.108, Vmin = 0.067; b The sequencing of eight candidate reference genes based on M value; c Scatter plots of NFs between V0.15 and Vmin-based scheme (r = Spearman rank correlation coefficient). d The comparison of target gene expression levels (CNRQ) using either the V0.15 or Vmin-based scheme. NF, normalization factor; CNRQ, calibrated normalized relative quantity

Back to article page