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Fig. 7 | BMC Biotechnology

Fig. 7

From: Impact of media components from different suppliers on enterokinase productivity in Pichia pastoris

Fig. 7

SDS-PAGE of the representative cleavage profile of the medium P3 Y3 G2 after fermentation. Lane 1: protein marker. Lane 2: substrate for enterokinase activity Trx-DCD1 as a control, Lane 3: substrate Trx-DCD1 cleaved with EKMax™, Lanes 4–10: Trx-DCD1 cleaved with undiluted medium, 100x diluted, 300x diluted, 900x diluted, 2700x diluted, 8100x, 24,300x diluted, respectively

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