Fig. 7
From: Simplified plasmid cloning with a universal MCS design and bacterial in vivo assembly
Additional versatility test for UMCS. a-e The efficiency of in vivo assembly according to 5 different UMCS-based vectors and 3 inserts was evaluated. All vector/insert combinations were repeated four times. For each transformation, 24 colonies were randomly picked for colony PCR and then subjected to agarose gel electrophoresis analysis. PCR products showing the same band as that of the insert were considered positive