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Table 5 Kinetic parameters of GsProS8 for casein and whey protein. Different letters (a-b) on the same column indicated significant (p < 0.05) differences among samples*

From: High level expression and biochemical characterization of an alkaline serine protease from Geobacillus stearothermophilus to prepare antihypertensive whey protein hydrolysate

Substrate

Vmax

(μmol/mg·min)

Km

(mg/mL)

kcat

(s−1)

kcat/Km

(mL/mg·s)

Casein

231.50 ± 3.07a

7.37 ± 0.24b

0.105

0.014

Whey protein

122.96 ± 4.98b

13.35 ± 1.17a

0.056

0.004

  1. *The measurement of kinetic parameters was carried out using casein and whey protein as substrates at 50 °C in 0.05 mol/L MOPS buffer (pH 8.5). Km and Vmax were shown as mean ± SD (n = 3)
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BMC Biotechnology

ISSN: 1472-6750

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