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Fig. 4 | BMC Biotechnology

Fig. 4

From: High level expression and biochemical characterization of an alkaline serine protease from Geobacillus stearothermophilus to prepare antihypertensive whey protein hydrolysate

Fig. 4

Optimal pH (a), pH stability (b), optimal temperature (c), and thermostability (d) of GsProS8. The influence of pH on GsProS8 activity was measured in 0.05 mol/L of different buffers at 40 °C. For the optimal pH, the relative activity (%) was determined against the specific activity of 3916 U/mg. To determine pH stability against the optimal activity (2631 U/mg, defined as 100% relative activity), GsProS8 was incubated in various buffers (0.05 mol/L) at 40 °C for 30 min. For optimal temperature, GsProS8 activity was tested at different temperatures in MOPS buffer (0.05 mol/L, at pH 8.5) in contrast to the specific activity of 3916 U/mg. The thermostability of GsProS8 was assayed against the optimal activity (3915 U/mg, defined as 100% relative activity) after incubation at different temperatures for 30 min

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