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Table 2 Independent variables and their coded levels chosen for BBD

From: Levan from Leuconostoc citreum BD1707: production optimization and changes in molecular weight distribution during cultivation

Variable code

Variable

Level

−1

0

+ 1

A

Cultivation time (h)

72

96

120

B

Cultivation temperature (°C)

20

25

30

C

Initial pH

5.5

6.5

7.5

D

Sucrose concentration (g/L)

100

150

200