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Fig. 1 | BMC Biotechnology

Fig. 1

From: Biobanked human foreskin epithelial cell sheets reduce inflammation and promote wound healing in a nude mouse model

Fig. 1

Epithelial cell culture and storage of ECSs. Primary epithelial cells (P0) (a) extracted from adult foreskin tissue. P1 epithelial cells show a typical paving stone-like morphology (b). Another 7 days, the epithelial cells were more compact (c) and continuous ECSs could be harvested. In the two-pump system (d), CPAs were pumped into the perfusion solution for mixing and then pumped into the ECS container. The DMSO concentration in the ECS container was slowly increased (e) until reaching 1.5 M. Subsequently, the ECSs were transferred to a pre-programmed freezer (f) and finally transferred to liquid nitrogen. Compared to the fresh controls, SEM demonstrated that cell membranes and intercellular connections ruptured in refrigerated groups(g). The frozen-2 group maintained an intact appearance and preserved most of the cell microstructure. Scar bar: A-C, 50 μm; Gross view, 1 cm; SEM, 20 μm

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