Table 1 Plasmids and fungal strain used in this study
From: Development of a CRISPR/Cpf1 system for targeted gene disruption in Aspergillus aculeatus TBRC 277
Plasmids/Strain | Genotype | Reference |
|---|---|---|
Plasmid | ||
 pFC902 | AmpR, pyrG, AMA1, U3-AF crRNA Cas9 | [21] |
 pJET1.2B-KmR | AmpR, kanR | This study |
 pJET-U3P sgRNA | AmpR, U3-AF sgRNA Cas9 | This study |
 pFC333 | AmpR, bleR, AMA1, TEF-Cas9 | [22] |
 pCRISPR0 | AmpR, bleR, AMA1 | This study |
 pCRISPR01 | AmpR, bleR, kanR, AMA1 | This study |
 pCRISPR01-eGFP | AmpR, bleR, kanR, AMA1, TEF1-EGFP | This study |
 pCRISPR01-FnCpf1 | AmpR, bleR, kanR, AMA1, TEF1-FnCpf1 | This study |
 pCRISPR01-FnCpf1-eGFP | AmpR, bleR, kanR, AMA1, TEF1-Cpf1-EGFP fusion | This study |
 pOK-U3P crRNA pyrG-1 | KanR, U3-AF crRNA-pyrG-1 | This study |
 pOK-U3P crRNA pyrG-2 | KanR, U3-AF crRNA-pyrG-2 | This study |
 pOK-U3P crRNA pyrG-3 | KanR, U3-AF crRNA-pyrG-3 | This study |
 pCRISPR01-FnCpf1-pyrG-1 | AmpR, bleR, kanR, AMA1, TEF-Cpf1, crRNA-pyrG-1 | This study |
 pCRISPR01-FnCpf1-pyrG-2 | AmpR, bleR, kanR, AMA1, TEF-Cpf1, crRNA-pyrG-2 | This study |
 pCRISPR01-FnCpf1-pyrG-3 | AmpR, bleR, kanR, AMA1, TEF-Cpf1, crRNA-pyrG-3 | This study |
Strain | ||
 A. aculeatus TBRC 277 | Wild type | [1] |